目的 建立HPLC-UV法測(cè)定金錢(qián)草中紫云英苷、山柰酚-3-O-蕓香糖苷、槲皮素、山柰酚和異鼠李素。方法 采用Eclipse XDB-C₁₈色譜柱（150 mm×4.6 mm，3.5 μm），流動(dòng)相為甲醇–0.4%磷酸水，梯度洗脫，檢測(cè)波長(zhǎng)360 nm，體積流量0.5 mL/min，柱溫30 ℃，進(jìn)樣量10 μL。結(jié)果 紫云英苷、山柰酚-3-O-蕓香糖苷、槲皮素、山柰酚、異鼠李素分別在22.10～176.80、21.14～169.12、17.40～174.00、51.12～408.96、23.30～186.40 μg/mL呈現(xiàn)良好線性關(guān)系（R²＞0.999 1）；平均加樣回收率分別為99.50%、100.39%、99.93%、105.58%、98.96%，RSD值分別為1.07%、1.44%、0.57%、0.24%、0.65%（n＝6）。結(jié)論 方法的重復(fù)性好，操作簡(jiǎn)單，結(jié)果準(zhǔn)確，可以用來(lái)控制金錢(qián)草藥材及其制劑的質(zhì)量。

Objective To develop an HPLC method for determination of astragalin, kaemperfol-3-O-rutinoside, quercetin, kaempferol, and isorhamnetin in Lysimachia christinae. Methods The analysis was performed on Eclipse XDB-C₁₈ column (150 mm × 4.6 mm, 3.5 μm). The mobile phase consisted of methanol and 0.4% phosphoric acid with gradient elution. The detection wavelength was 360 nm, flow rate was 0.5 mL/min, column temperature was 30 ℃, and volume of injection was 10 μL. Results Aastragalin, kaemperfol-3-O-rutinoside, quercetin, kaempferol, and isorhamnetin showed good linear relationships of 22.10 — 176.80, 21.14 — 169.12, 17.40 — 174.00, 51.12 — 408.96, and 23.30 — 186.40 μg/mL (R² ≥ 0.999 1), whose average recoveries were 99.50%, 100.39%, 99.93%, 105.58%, and 98.96% with RSD values of 1.07%, 1.44%, 0.57%, 0.24%, and 0.65% (n＝6), respectively. Conclusion The method is reproducible, simple, and accurate, which can be used for the quality control of Lysimachia christinae and its preparations.

R286.02

國(guó)家自然科學(xué)基金資助項(xiàng)目（81760701）