目的 研究植物乳桿菌RG-034的體外抑菌效果以及對(duì)大鼠腹瀉型腸易激綜合征的治療作用。方法 采用活菌計(jì)數(shù)法測(cè)定RG-034菌粉活菌數(shù),利用類(lèi)"牛津杯法"確定植物乳桿菌RG-034對(duì)大腸埃希菌、乙型副傷寒沙門(mén)菌、金黃色葡萄球菌的抑菌活性。SD大鼠按體質(zhì)量隨機(jī)分為對(duì)照組、模型組、蒙脫石散組及植物乳桿菌RG-034膠囊組,每組6只。除對(duì)照組外,其余大鼠每日ig 5 g/kg番瀉葉聯(lián)合隔日禁食,持續(xù)5周,制備大鼠腹瀉型腸易激綜合征模型。自造模第3周開(kāi)始給藥,植物乳桿菌RG-034組大鼠ig RG-034膠囊(1×10⁹ CFU/粒,15 mg),2粒/d;蒙脫石散組大鼠ig 0.81 g/kg蒙脫石散,1次/d;對(duì)照組和模型組大鼠ig等量生理鹽水,給藥3周。觀(guān)察大鼠一般狀態(tài)變化,測(cè)定稀便率、稀便級(jí)以及腹瀉指數(shù),采用16S核糖體RNA測(cè)序技術(shù)分析大鼠結(jié)腸中腸道菌群變化。結(jié)果 植物乳桿菌RG-034菌粉活菌數(shù)為2.5×10¹¹ CFU/g,對(duì)不同濃度的大腸埃希菌、乙型副傷寒沙門(mén)菌、金黃色葡萄球菌均有一定的抑制作用,抑菌圈直徑為15.90~25.06 mm。與模型組比較,植物乳桿菌RG-034膠囊可明顯改善大鼠腹瀉情況,顯著降低稀便率、稀便級(jí)以及腹瀉指數(shù),增加了腸道菌群多樣性。結(jié)論 植物乳桿菌RG-034對(duì)大鼠腹瀉型腸易激綜合征具有一定的治療作用,其機(jī)制可能與調(diào)節(jié)腸道菌群多樣性有關(guān)。

Objective To investigate the bacteriostatic effect of Lactobacillus plantarum RG-034 in vitro and its therapeutic effect on the diarrhoea-predominant irritable bowel syndrome in rats. Methods The viable bacteria count of RG-034 powder was determined by using the "Oxford Cup method" to determine the antibacterial activity of Lactobacillus plantarum RG-034 against Escherichia coli, Salmonella paratyphi B and Staphylococcus aureus. SD rats were randomly divided into control group, model group, Montmorillonite Powder group, and Lactobacillus plantarum RG-034 capsule group by body weight, with 6 rats in each group. Except the control group, other rats were given 5 g/kg senna leaf combined with alternate day fasting daily for 5 weeks to prepare a rat model of diarrhea-type irritable bowel syndrome. At the 3rd week of self-modeling, rats in Lactobacillus plantarum RG-034 group were given RG-034 Capsules (1 × 10⁹ CFU/Capsule, 15 mg), 2 capsules/d. Rats in Montmorillonite Powder group were ig 0.81 g/kg Montmorillonite Powder once daily. The rats in control group and model group were given the same amount of normal saline intragaically for 3 weeks. The changes of the general status of rats were observed, the rate of loose stool, the grade of loose stool and the diarrhea index were determined, and the changes of intestinal flora in the colon of rats were analyzed by 16S ribosomal RNA sequencing technology. Results The viable bacteria number of Lactobacillus plantarum RG-034 Powder was 2.5 × 10¹¹ CFU/g, which had certain inhibitory effect on Escherichia coli, Salmonella paratyphi B and Staphylococcus aureus at different concentrations, and the inhibitory zone diameter was 15.90-25.06 mm. Compared with the model group, Lactobacillus plantarum RG-034 Capsules can significantly improve the diarrhea situation of rats, significantly reduce the rate of loose stool, loose stool grade and diarrhea index, and increase the diversity of intestinal flora. Conclusion Lactobacillus plantarum RG-034 has a certain therapeutic effect on diarrhea-predominant irritable bowel syndrome in rats, and its mechanism may be related to the regulation of intestinal microbiota diversity.

R965