目的 建立指紋圖譜和多指標成分測定方法評價不同產(chǎn)地廣東王不留行藥材。方法 采用UPLC法建立廣東王不留行藥材特征圖譜和綠原酸、蘆丁的測定方法,采用熵權法所得權重作為分辨系數(shù)構建廣東王不留行的質量評價模型,灰色關聯(lián)度法計算各指標的相關關聯(lián)度。結果 廣東王不留行UPLC特征圖譜共確定8個共有峰,指認了其中3個成分新綠原酸、綠原酸、隱綠原酸。測定了13批不同產(chǎn)地廣東王不留行中綠原酸和蘆丁。13批廣東王不留行藥材的相對關聯(lián)度范圍為0.116 2~0.810 5,不同批次樣品之間的質量存在一定的差異,廣東肇慶產(chǎn)廣東王不留行的質量較優(yōu)。結論 建立的方法可有效評價不同產(chǎn)地廣東王不留行的質量,為廣東王不留行的藥材質量控制提供參考。

Objective To evaluate the crude drug of Fici Pumilae Receptaculum from different origins by characteristic chromatogram and multiple index. Methods UPLC was used to establish the characteristic chromatogram and the determination of chlorogenic acid and rutin in Fici Pumilae Receptaculum, and the quality evaluation model of Fici Pumilae Receptaculum was established by using the entropy weight method as the resolution coefficient. The correlation between various indicators was calculated by grey relation analysis. Results Eight common peaks were established in UPLC characteristic chromatogram, and that 3 of them were identified as neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid. Contents of chlorogenic acid and rutin in 13 batches of Fici Pumilae Receptaculum from different origins were determined. The relative correlation degree of 13 batches of samples ranged from 0.116 2 to 0.810 5. There were certain differences in quality between different batches of samples, and Fici Pumilae Receptaculum samples from Zhaoqing City in Guangdong Province was better. Conclusion The method can effectively evaluate the quality of Fici Pumilae Receptaculum from different origins, providing reference for the quality control of Fici Pumilae Receptaculum crude drug.

R286.02

國家工信部產(chǎn)業(yè)技術基礎公共服務平臺項目（2022-230-221）；佛山市南海區(qū)重點領域科技攻關專項（南科[2023]20號-18）