目的 采用多種實(shí)驗(yàn)?zāi)Ｐ驮u(píng)價(jià)痛風(fēng)康寧片防治痛風(fēng)性關(guān)節(jié)炎的有效性。方法 采用微晶型尿酸鈉（MSU）誘導(dǎo)大鼠急、慢性痛風(fēng)性關(guān)節(jié)炎模型，觀察痛風(fēng)康寧片對(duì)急、慢性痛風(fēng)性關(guān)節(jié)炎的預(yù)防和治療作用；采用大鼠棉球肉芽腫模型，觀察痛風(fēng)康寧片的抗炎作用；采用血瘀大鼠模型，觀察痛風(fēng)康寧片的活血作用；采用小鼠醋酸扭體模型，觀察痛風(fēng)康寧片的鎮(zhèn)痛作用。結(jié)果 與模型組比較，痛風(fēng)康寧片預(yù)防給藥，1.15、2.3 g/kg能明顯減輕MSU引起的大鼠右踝關(guān)節(jié)腫脹、改善步態(tài)、減少血清中白細(xì)胞介素（IL）-1β、IL-6含量；痛風(fēng)康寧片治療給藥，1.15、2.3 g/kg能明顯減輕MSU引起的大鼠右踝關(guān)節(jié)腫脹、改善步態(tài)；痛風(fēng)康寧片1.15、2.3 g/kg明顯降低慢性關(guān)節(jié)炎大鼠氣囊灌洗液中IL-1β、IL-6、腫瘤壞死因子-α（TNF-α）含量和吞噬細(xì)胞數(shù)；痛風(fēng)康寧片1.15、2.3 g/kg能明顯減輕大鼠棉球肉芽重量；痛風(fēng)康寧片0.58、1.15、2.3 g/kg明顯抑制血瘀大鼠全血粘度的升高；痛風(fēng)康寧片1.66、3.33 g/kg組明顯減少醋酸誘導(dǎo)的小鼠扭體次數(shù)。結(jié)論 痛風(fēng)康寧片有明顯的抗炎、鎮(zhèn)痛及活血作用，對(duì)痛風(fēng)性關(guān)節(jié)炎有明顯的治療作用。

Objective To evaluate the effectiveness of Tongfeng Kangning Tables in prophylactic and therapeutic of gouty arthritis in various experimental models. Methods To observe the preventive and therapeutic effects of Tongfeng Kangning Tables on acute and chronic gouty arthritis in rats induced by microcrystalline sodium urate (MSU). The anti-inflammation effect of Tongfeng Kangning Tables was observed by using cotton ball granuloma model in rats. Blood stasis rat model was used to observe the blood-activating effect of Tongfeng Kangning Tables. The analgesic effect of gout Tongfeng Kangning Tables was observed by using acetic acid writhing model in mice. Results Compared with model group, prophylactic administration of Tongfeng Kangning Tables in 1.15 and 2.3 g/kg groups could significantly reduce the swelling of right ankle joint induced by MSU, improve gait, and reduce the contents of IL-1β and IL-6 in serum. Tongfeng Kangning Tables in 1.15 and 2.3 g/kg groups could significantly reduce the swelling of the right ankle and improve the gait induced by MSU. The contents of IL-1β, IL-6, tumor necrosis factor-α (TNF-α) and the number of phagocytic cells in the balloon lavage fluid of chronic arthritis rats were significantly reduced in the 1.15 and 2.3 g/kg Tongfeng Kangning Tables groups. Tongfeng Kangning Tables in 1.15 and 2.3 g/kg groups could significantly reduce the weight of cotton ball granulation. Tongfeng Kangning Tables in 0.58, 1.15, and 2.3 g/kg groups significantly inhibited the increase of whole blood viscosity of blood stasis rats. Tongfeng Kangning Tables 1.66 and 3.33 g/kg groups significantly reduced the number of body twisting induced by acetic acid. Conclusion Tongfeng Kangning Tables has obvious anti-inflammatory, analgesic and blood-activating effects, and has obvious therapeutic effects on gouty arthritis.

R285.5

國(guó)家科技支撐計(jì)劃課題（2013BAI09B01）