目的 采用化學(xué)計量學(xué)、加權(quán)TOPSIS與灰色關(guān)聯(lián)度融合模型評價不同產(chǎn)地獨腳金的質(zhì)量差異。方法 采用HPLC法測定獨腳金中木犀草素、芹菜素、金圣草素、香葉木素、金合歡素、毛蕊花糖苷、異毛蕊花糖苷、桃葉珊瑚苷、β-谷甾醇，同時檢測水溶性浸出物、醇溶性浸出物、總灰分和酸不溶性灰分，對結(jié)果進(jìn)行PCA和OPLS-DA分析，挖掘影響產(chǎn)品質(zhì)量的差異性標(biāo)志物。建立加權(quán)TOPSIS與GRA融合模型，對不同產(chǎn)地獨腳金質(zhì)量進(jìn)行綜合評價。結(jié)果 18批獨腳金批次間差異較大，PCA結(jié)果顯示18批獨腳金聚為3類，OPLS-DA分析從13個變量中篩選出4個差異性標(biāo)志物。加權(quán)TOPSIS與灰色關(guān)聯(lián)度融合模型顯示18批樣品的綜合相對貼近度在0.293 1～0.665 8，以S14綜合相對貼近度最高。結(jié)論 所建方法分析用時短，結(jié)果準(zhǔn)確，可用于獨腳金多指標(biāo)成分定量控制和質(zhì)量評價。

Objective To evaluate the quality difference of Striga asiatica from different producing areas by chemometrics, weighted TOPSIS, and grey correlation degree fusion model. Methods The contents of luteolin, apigenin, chrysoeriol, diosmetin, acacetin, verbascoside, isoacteoside, aucubin and β-sitosterol in S. asiatica were simultaneously determined by HPLC. At the same time, the water-soluble extract, alcohol-soluble extract, total ash, and acid-insoluble ash were detected. PCA and OPLS-DA were used to analyze the quantitative detection results, and the differential markers affecting product quality were excavated. A weighted TOPSIS and GRA fusion model was established to comprehensively evaluate the quality of S. asiatica from different producing areas. Results There was a large difference among 18 batches of S. asiatica. PCA results showed that S. asiatica were clustered into 3 categories. OPLS-DA analysis screened 4 differential markers from 13 variables. The fusion model of weighted TOPSIS and grey correlation degree showed that the comprehensive relative closeness of 18 batches of samples was between 0.293 1 and 0.665 8, and the comprehensive relative closeness of S14 was the highest. Conclusion The established method is convenient and accurate, and can be used for quantitative control and quality evaluation for S. asiatica.

R286.02

國家中醫(yī)藥管理局全國中藥特色技術(shù)傳承人才培訓(xùn)項目[國中醫(yī)藥人教函（2023）96號]