目的 采用HPLC法同時(shí)測(cè)定解郁安神顆粒中梔子苷、異阿魏酸、阿魏酸、甘草酸和柴胡皂苷b₂。方法 使用Agilent Zorbax Eclipse XDB-C₁₈色譜柱（150 mm×4.6 mm，5 μm）；流動(dòng)相0.2%磷酸水-乙腈，梯度洗脫；檢測(cè)波長(zhǎng)238 nm；體積流量1 mL/min；柱溫35 ℃；進(jìn)樣量10 µL。結(jié)果 梔子苷、異阿魏酸、阿魏酸、甘草酸、柴胡皂苷b₂的線性范圍良好，平均回收率分別為105.33%、103.07%、101.36%、99.61%、100.00%，RSD值分別為0.69%、2.30%、2.44%、1.28%、1.28%。結(jié)論 檢測(cè)結(jié)果準(zhǔn)確，檢測(cè)范圍較廣，適用于解郁安神顆粒的質(zhì)量評(píng)價(jià)研究。

Objective To adopt an HPLC method to simultaneously determine the contents of geniposide, isoferulic acid, ferulic acid, glycyrrhizic acid, and saikosaponin b₂ in Jieyu Anshen Granules.Methods Agilent Zorbax Eclipse XDB-C₁₈ chromatographic column (150 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of 0.2% phosphoric acid water and acetonitrile with gradient elution. Detection wavelength was 238 nm, flow rate was 1 mL/min, column temperature was 35℃, and injection volume was 10 μL. Results Geniposide, isoferulic acid, ferulic acid, glycyrrhizic acid, and saikosaponin b₂ had good specificity, and their recovery rates were 105.33%, 103.07%, 101.36%, 99.61%, and 100.00%, with RSD values of 0.69%, 2.30%, 2.44%, 1.28%, and 1.28%, respectively. Conclusion The detection results of this experiment are accurate and have a wide detection range, which is suitable for the quality evaluation for Jieyu Anshen Granules.

R286.02

江西省中醫(yī)藥管理局科技計(jì)劃項(xiàng)目（2023B1357）