目的 建立龍脷葉多成分定量控制方法，采用化學(xué)模式識(shí)別評(píng)價(jià)不同產(chǎn)地龍脷葉質(zhì)量差異。方法 采用HPLC法同時(shí)測(cè)定龍脷葉中原兒茶酸、咖啡酸、6-羥基香豆素、金絲桃苷、異槲皮苷、槲皮素、山柰酚、山柰酚-3-O-龍膽二糖苷、豆甾醇、β-谷甾醇，并檢測(cè)浸出物、總灰分和酸不溶性灰分。采用聚類(lèi)分析（CA）、主成分分析（PCA）、因子分析（FA）和偏最小二乘回歸分析（PLS-DA）法對(duì)18批不同產(chǎn)地的龍脷葉進(jìn)行區(qū)分比較，尋找差異因子。結(jié)果 18批龍脷葉樣品中各成分含量差異較大，明顯分為3類(lèi)，廣東和廣西產(chǎn)地的龍脷葉整體質(zhì)量較好。山柰酚-3-O-龍膽二糖苷、咖啡酸、金絲桃苷、原兒茶酸、6-羥基香豆素和異槲皮苷可作為龍脷葉的質(zhì)量差異標(biāo)記物。結(jié)論 建立的多指標(biāo)成分定量、化學(xué)模式識(shí)別可評(píng)價(jià)龍脷葉質(zhì)量，為龍脷葉的質(zhì)量控制和道地性研究提供參考。

Objective To establish a multi-components quantitative analysis method for Sauropi Folium, and to evaluate the quality difference of Sauropi Folium from different habitats by chemical pattern recognition methods. Methods The contents of protocatechuic acid, caffeic acid, 6-hydroxycoumarin, hyperoside, isoquercitrin, quercetin, kaempferol, kaempferol-3-O-gentionbioside, stigmasterol, and β-sitosterol in Sauropi Folium were simultaneously determined by HPLC method. Extract, total ash, and acid insoluble ash were also detected. The CA, PCA, FA, and PLS-DA were used to distinguish and compare 18 batches of Sauropi Folium from different habitats, and explore the difference factors for quality evaluation of Sauropi Folium. Results There were significant differences in the content of various components in 18 batches of Sauropi Folium. 18 Batches of Sauropi Folium were clustered into 3 categories, and the quality of Sauropi Folium from Guangdong and Guangxi Province was better. Kaempferol-3-O-gentionbioside, caffeic acid, hyperoside, protocatechuic acid, 6-hydroxycoumarin, and isoquercitrin could be used as quality difference markers for Sauropi Folium. Conclusion The established multi-index component quantitative analysis and chemical pattern recognition can evaluate the quality of Sauropi Folium, and it provides reference for quality control and genuineness research of Sauropi Folium.

R286.02

安徽省教育廳高校優(yōu)秀拔尖人才培育資助項(xiàng)目（gxbjZD2020061）