目的 建立水紅花子中多指標(biāo)成分定量，結(jié)合化學(xué)計量學(xué)評價不同產(chǎn)地水紅花子的質(zhì)量。方法 采用HPLC法測定水紅花子中原兒茶酸、兒茶素、異葒草素、葒草素、花旗松素、木犀草素、槲皮素、山柰酚、圣草酚、白樺脂酸和β-谷甾醇，并檢測醇溶性浸出物、總灰分和酸不溶性灰分，采用主成分分析、因子分析、正交偏最小二乘法-判別分析、熵權(quán)-TOPSIS對檢測結(jié)果分析。結(jié)果 18批水紅花子樣品自動聚為3類，呈現(xiàn)地域性分布。兒茶素、花旗松素、原兒茶酸、槲皮素、山柰酚、β-谷甾醇和異葒草素7個成分可作為引起不同產(chǎn)地水紅花子之間質(zhì)量差異的主要變量，是質(zhì)量差異標(biāo)志物。黑龍江、遼寧和吉林產(chǎn)地的水紅花子整體質(zhì)量較好，其中遼寧西豐縣的樣品質(zhì)量最優(yōu)，廣西和四川產(chǎn)的水紅花子質(zhì)量較差。結(jié)論 所建立的多組分定量和化學(xué)計量學(xué)控制模式簡單、易推廣，可用于水紅花子的內(nèi)在質(zhì)量評價。

Objective To establish an HPLC multi-index component quantification method for Polygoni Orientalis Fructus, and to evaluate the quality of Polygoni Orientalis Fructus from different areas by chemometrics. Methods Simultaneous determination of protocatechuic acid, catechin, isoorientin, orientin, taxifolin, luteolin, quercetin, kaempferol, eriodictyol, betulinic acid, and β- sitosterol in Polygoni Orientalis Fructus were carried on by HPLC method. Alcohol-soluble extractive, total ash, and acid-insoluble ash were also detected. PCA, FA, OPLS-DA, and EW-TOPSIS methods were used to analyze the results. Results 18 Batches of Polygoni Orientalis Fructus samples were automatically clustered into 3 categories, showing a regional distribution. Seven components, including catechins, taxifolin, protocatechuic acid, quercetin, kaempferol, β-sitosterol, and isoorientin, could serve as the main variables causing quality differences in Polygoni Orientalis Fructus from different areas and were indicators of quality differences. The overall quality of samples from Heilongjiang, Liaoning, and Jilin Province production areas were good, with the sample quality from Xifeng County, Liaoning Province being the best, while the samples from Guangxi and Sichuan Province had poor quality. Conclusion The multi-index component quantification method and chemometric control mode established is simple and easy to generalize, and can be used for the internal quality evaluation for Polygoni Orientalis Fructus.

R286.02

河南省高等學(xué)校重點科研指導(dǎo)計劃項目（20B550007）