目的 建立越鞠保和丸UPLC指紋圖譜，采用多元數(shù)據(jù)統(tǒng)計方法分析評價不同企業(yè)產品質量。方法 采用UPLC法建立越鞠保和丸的指紋圖譜，確定共有峰，進行相似度的評價，采用聚類分析、主成分分析、正交偏最小二乘法-判別分析綜合分析越鞠保和丸質量。結果 越鞠保和丸的UPLC指紋圖譜的相似度均大于0.90。共確定了16個共有峰，指認了其中7個色譜峰。越鞠保和丸樣品分為3類，4個主成分累積方差貢獻率為81%，篩選出5個共有峰VIP＞1的化合物，依次為峰9（西紅花苷Ⅰ）、峰3（梔子苷）、峰13（藁本內酯）、峰16（蒼術素）、峰4（阿魏酸），這些成分是影響越鞠保和丸質量差異的標志性成分。結論 UPLC指紋圖譜結合多元數(shù)據(jù)統(tǒng)計可作為評價越鞠保和丸質量的方法。

Objective To establish the UPLC fingerprint and evaluate quality of Yueju Baohe Pills from different manufacturers using multivariate statistical methods. Methods Fingerprints of Yueju Baohe Pills were established using UPLC method, common peaks were identified, and similarities were evaluated. The quality of Yueju Baohe Pills was comprehensively analyzed using HCA, PCA, and OPLS-DA. Results The similarities of Yueju Baohe Pills UPLC fingerprints were above 0.90. A total of 16 common peaks were identified, of which 7 chromatographic peaks were identified. The samples of Yueju Baohe Pills were divided into three categories, and the cumulative variance contribution rate of the four principal components was 81%. Five compounds with a common peak VIP > 1 were screened, namely peak 9 (crocin I), peak 3 (geniposide), peak 13 (pyrolide), peak 16 (atractylodes), and peak 4 (ferulic acid), which were characteristic components affecting the quality differences of Yueju Baohe Pills. Conclusion UPLC fingerprints combined with multivariate statistical methods can be used as a method for evaluating the quality of Yueju Baohe Pills.

R286.02