目的 對db/db小鼠進行生物學(xué)特性研究，滿足糖尿病研究的需要。方法 在試驗周期內(nèi)，測定db/db小鼠的空腹血糖、體質(zhì)量及攝食飲水量，并測定21周齡模型動物的糖耐量、血中胰島素、胰高血糖素、三酰甘油、總膽固醇等指標的水平，進行胰腺的免疫組化雙重染色及主要臟器的病理學(xué)檢查，并與db/m小鼠進行比較。結(jié)果 與對照組db/m小鼠比較，db/db小鼠過度肥胖，伴有高血糖、胰島素抵抗、脂質(zhì)代謝紊亂，且肝臟和胰腺組織均出現(xiàn)明顯病變，胰腺免疫組化雙重染色結(jié)果與血液學(xué)測定結(jié)果一致，并可觀察到胰島中A細胞和B細胞的分布變化情況。結(jié)論 對自發(fā)性2型糖尿病模型db/db小鼠所做的相關(guān)生物學(xué)特性檢測，可供糖尿病研究參考。

To investigate the biological characteristics of db/db mice to meet the need of study on diabetes mellitus. Methods In this experiment, the fasting blood glucose, body weight, and food and water intake were measured. The carbohydrate tolerance, insulin, glucagon, triglyceride, and total cholesterol in serum were tested on db/db mice of 21 weeks. Meanwhile, the immunohistochemical double staining in pancreas and morphological study was carried out. All the data were compared with db/m mice in statistics. Results Compared with db/m mice, obesity, hyperinsulinemia, hyperglycemia, and lipid metabolism disorder were serious on db/db mice, following by obvious pathological deterioration of liver and pancreas. According to the result of blood testing, the changes in the distribution of A cells and B cells could be observed in immunohistochemical double staining on pancreas of db/db mice. Conclusion The db/db mice could be used as appropriate disease model of type 2 diabetes mellitus, providing relevant biological information for further study in this field.