目的：研究藻紅蛋白對人乳腺癌 MCF-7 細(xì)胞的誘導(dǎo)衰老作用。方法：采用 MTT 法檢測藻紅蛋白對 MCF-7 細(xì)胞的生長抑制作用，采用倒置顯微鏡觀察形態(tài)學(xué)變化，酸性 β-半乳糖苷酶染色法和衰老相關(guān)異染色質(zhì)聚集檢測藻紅蛋白誘導(dǎo)人乳腺癌 MCF-7 細(xì)胞衰老作用。結(jié)果：MTT 法結(jié)果顯示，藻紅蛋白對體外培養(yǎng)的人乳腺癌細(xì)胞 MCF-7 具有明顯的生長抑制作用，IC₅₀ 為 134.87 μg/mL。結(jié)果發(fā)現(xiàn)藻紅蛋白作用 72 h 后，細(xì)胞呈現(xiàn)變大變扁平，細(xì)胞內(nèi)形成空泡、顆粒增多等明顯衰老形態(tài)。在倒置顯微鏡下觀察，酸性 β-半乳糖苷酶染色呈陽性；在熒光顯微鏡下觀察，DAPI 染色出現(xiàn)衰老相關(guān)異染色質(zhì)聚集現(xiàn)象。結(jié)論：藻紅蛋白可通過誘導(dǎo) MCF-7 細(xì)胞衰老達(dá)到抗腫瘤作用。

Objective: To investigate the effects of phycoerythrin（PE）on the induction of cell senescence in human breast cancer MCF-7 cells. Methods: The inhibitory rate of MCF-7 cells growth was measured by MTT assay and morphological changes of MCF-7 cells was observed through the inverted microscope. Acidic β-galactosidase dying (SA-β-Gal dying) and senescence-associated heterochromatin aggregation were used to detect the cell senescence caused by PE in MCF-7 cells. Results: The results of MTT assay indicates that PE could inhibit the growth of human breast cancer MCF-7 cells significantly and the IC₅₀ was 134.87 μg/mL. After 72 h, MCF-7 cells which were treated by PE had become bigger and flatter meanwhile the appearance of vacuolization and particles which are the specific patterns of the cell senescence were also observed. The staining of acidic β-galactosidase was positive, which observed by inverted microscope, and, the emergence of senescence-associated heterochromatin aggregation (SAHF) in staining of DAPI was found under the fluorescence microscope.Conclusion: The results demonstrate that PE has the antitumor effect by inducing the cell senescence of MCF-7 cells.

國家自然科學(xué)基金項(xiàng)目（300672595）；教育部科學(xué)技術(shù)研究重點(diǎn)項(xiàng)目（207030）；教育部博士點(diǎn)基金項(xiàng)目（20070240081）；黑龍江省自然科學(xué)基金重大項(xiàng)目（ZJY0504）