TM(鉆石)C18 色譜柱(200 mm × 4.6 mm,5 μm),流動相為乙腈 - 水(65︰35),柱溫 25 ℃,體積流量 1.0 mL/min,檢測波長為 230 nm,魚精蛋白凝聚法分離游離藥物,測定復方脂質體中洛莫司汀的含量及包封率;使用 Diamonsil TMC18 色譜柱(200 mm × 4.6 mm,5 μm),流動相為甲醇 - 水(10︰90),柱溫 25 ℃,體積流量 1.0 mL/min,檢測波長為 244 nm,魚精蛋白凝聚法分離游離藥物,測定復方脂質體中碘海醇的含量及包封率。結果:洛莫司汀與輔料及溶劑峰分離良好,在 1.0~20.0 μg/mL線性關系良好(r = 1.0, n = 5),回收率為 99.0 %~101.0 %;碘海醇與輔料及溶劑峰分離良好,在 6.0~60.0 μg/mL線性關系良好(r = 0.999 9, n = 5),回收率為99.0 %~101.0 %。結論:該方法準確、簡單,可用于洛莫司汀-碘海醇復方脂質體含量及包封率的測定。;Objective: To establish an HPLC method for determining the content and entrapment efficiency of lomustine-iohexol compound liposomes. Methods: The separation was performed with a Diamonsil TM C18 column(200 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile-water(65∶35), the drug was detected at 230 nm wavelength and the flow rate was 1.0 mL/min, with column temperature of 25℃, protamine aggregation method was applied to separating the free drug and liposomes, for determining the content and entrapment efficiency of lomustine;the separation was performed with a DiamonsilTM C18 column(200 mm × 4.6 mm, 5 μm), the mobile phase was methanol-water(10∶90), the drug was detected at 244 nm wavelength and the flow rate was 1.0 mL/min, with column temperature of 25 ℃, protamine aggregation method was applied to separating the free drug and liposomes, for determining the content and entrapment efficiency of iohexol. Results: Lomustine and iohexol can be well separated with a good linear relationship in the rages of 1.0 — 20.0 μg/mL(r = 1.0, n = 5)and 6.0 — 60.0 μg/mL(r = 0.999 9, n =5), their average recoveries were both between 99.0 % — 101.0 %, respectively. Conclusion: This method is accurate and simple, and can be well used to determine the contentand entrapment efficiency of lomustine-iohexol compound liposomes."/>

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首頁 > 過刊瀏覽>2009年第32卷第1期 >2009,32(1):38-42. DOI:10.7501/j.issn.0253-6376.[year].1.[sequence]
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HPLC 法測定洛莫司汀-碘海醇復方脂質體的藥物含量及包封率

Determination of content and entrapment efficiency of lomustine-iohexol compound liposomes by HPLC

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