目的：比較狹窄冠狀動脈導(dǎo)致心肌缺血模型犬在麻醉和清醒狀態(tài)下的差異，及藥物治療的差異。方法：在無菌條件下給犬心外膜安置心電電極、血壓導(dǎo)管、信號發(fā)射器并分離冠狀動脈前降支，放置動脈壓迫環(huán)。手術(shù)恢復(fù)期后，動物按正交設(shè)計給予處置：分別在清醒與麻醉狀態(tài)下狹窄冠狀動脈，進行模型組和硝酸甘油治療組的觀察。主要監(jiān)測心外膜心電圖ST段、血壓、心率、體溫。結(jié)果與結(jié)論：麻醉犬心率明顯高于清醒犬，冠脈狹窄后麻醉及清醒犬心電圖ST段均明顯升高；硝酸甘油治療的效果，清醒犬較麻醉犬起效快，維持時間長。

Objective: Using model of myocardial ischemia by coronary artery stenosis with artery rings, the difference between anesthetized and conscious dogs, as well as drug treatment, was compared. Methods: Under sterile conditions, electrode of epicardial ECG, catheter of blood pressure, and the signal transmitter were imbedded in Beagle dogs, and the artery rings were placed on anterior descending coronary artery. After surgery, the coronary artery stenosis was made in anesthetized or conscious state according to orthogonal design, the therapeutic effect of nitroglycerin or vehicle was observed. Data of epicardial ECG ST-segment, blood pressure, heart rate, and body temperature were acquired and analyzed. Results and Conclusion: Heart rate was higher in anesthetic dogs than that in conscious dogs. Epicardial ECG ST-segment was increased both in conscious and anesthetic dogs significantly after coronary artery stenosis. Therapeutic effects of nitroglycerin provide faster onset of action and longer time in conscious dogs compared with anesthetic dogs.

國家科技支撐計劃項目（NO.2007BAI41B02）、系列化、國際化的國家生物醫(yī)藥國際創(chuàng)新園新藥研發(fā)綜合性大平臺建設(shè)項目（2009ZX09301-008）