目的：研究五加苷對(duì)大鼠局灶性腦梗死的防治及對(duì)腦缺血模型大鼠腦梗死灶體積的影響。方法：對(duì)局灶性腦梗死的防治，采用栓線法造模，檢測(cè)各組大鼠腦組織含水量，并觀察腦組織形態(tài)學(xué)變化；對(duì)腦缺血模型大鼠腦梗死灶體積影響的研究，依據(jù)Zea-Longa栓線法造模，治療組以五加苷ig，對(duì)照組用生理鹽水代替五加苷，Swanson法測(cè)定腦梗死灶體積，并觀察腦組織形態(tài)學(xué)變化。結(jié)果：經(jīng)五加苷治療的局灶性腦梗死大鼠的腦含水量降低，光學(xué)顯微鏡下觀察腦組織形態(tài)學(xué)有明顯改善；經(jīng)五加苷治療的腦缺血模型大鼠腦梗死灶體積顯著降低。結(jié)論：五加苷對(duì)腦梗死大鼠有明顯腦保護(hù)作用。

Objective: To study the prevention and treatment of cerebral infarction in rats using acankoreoside A, and the effect of acankoreoside A on cerebral infarction volume in focal cerebral ischemia rat model. Methods: For the research of the prevention and treatment of focal cerebral infarction, the models of the focal cerebral infarction of the rats were made by putting the special thread to the internal carotid artery. The water content in the rats’brain was detected, and the brain morphological changes were observed. For the study of the effect of acankoreoside A on cerebral infarction volume in focal cerebral ischemia rat model, the models of the focal cerebral infarction rats of the treated group and normal group were made according to Zea-Longa modeling filament method. The treated group rats were administrated with acankoreoside A and the normal group rats with normal saline. The cerebral infarction volumes were determined by Swanson method and the brain morphological changes were observed. Results: The results show that that the water content in the focal cerebral infarction rats’ brain is significantly reduced, brain morphology is obviously improved though optical microscope observation, and cerebral ischemia infarct volume of focal cerebral ischemia rats is distinctly lower after treated with acankoreoside A. Conclusion: Acankoreoside A has significantly protective effect on cerebral infarction in rats.

教育部留學(xué)回國(guó)人員科研啟動(dòng)基金（教外司留[2005]55號(hào)）；湖南省教育廳中藥學(xué)重點(diǎn)學(xué)科和湖南中醫(yī)藥大學(xué)十一五校級(jí)重點(diǎn)學(xué)科中藥化學(xué)與分析資助。