目的：研究清肺消炎丸對豚鼠的鎮(zhèn)咳平喘作用。方法：采用枸櫞酸誘發(fā)豚鼠咳嗽模型，記錄引咳后3 min內(nèi)的咳嗽次數(shù)，觀察清肺消炎丸的鎮(zhèn)咳作用。采用豚鼠離體氣管平滑肌收縮實(shí)驗(yàn)和組胺引喘的活體動(dòng)物實(shí)驗(yàn)對清肺消炎丸的平喘作用進(jìn)行評價(jià)。離體實(shí)驗(yàn)中測定藥物對乙酰膽堿引起的氣管平滑肌收縮的收縮曲線，計(jì)算收縮百分率；整體動(dòng)物實(shí)驗(yàn)中測定給藥后豚鼠的延喘時(shí)間用來表征藥物平喘作用的強(qiáng)弱。結(jié)果：清肺消炎丸能有效減少豚鼠的咳嗽次數(shù)，顯著延長咳嗽潛伏期；對豚鼠離體氣管收縮的抑制效果較好；對組胺致喘豚鼠的哮喘潛伏期有明顯的延長作用。結(jié)論：清肺消炎丸具有良好的鎮(zhèn)咳、平喘藥理活性，為藥物的臨床應(yīng)用和進(jìn)一步開發(fā)提供了依據(jù)。

Objective: To study antitussive and anti-asthmatic effect of Qingfeixiaoyan Pill in guinea pigs. Methods: The aminal cough model induced by citric acid was developed to evaluate the antitussive effect. Qualified guinea pigs were divided into five groups randomly: high, medium, and low concentration Qingfeixiaoyan Pill groups, pentoxyverine positive control group, and control group. The number of cough during three minutes was recorded after the cough was induced. Meanwhile, guinea pig models of isolated trachea in vitro and hitamine-induced asthma in vivo were used to investigate the anti-asthmatic effect of Qingfeixiaoyan Pill. In vitro experiment, concentration-response curves of contractile isolated trachea induced by acetylcholine (Ach) were obtained and the concentration producing 50% of the maximal response was determined. In vivo experiment, the latent period of animals after dosage was uesd to demonstrate the anti-asthmatic effect. Results: Qingfeixiaoyan Pill decreased the frequency of cough and prelonged the latency of induced cough. Morever, it significantly inhibited Ach-induced trachea contractions and prelonged the latent period of asthma by hitamine in guinea pigs. Conclusion: Qingfeixiaoyan Pill has remarkable antitussive and anti-asthmatic effects, which provides the evidence for clinical application and further development.