目的：冰片和薄荷腦是清咽滴丸中主要揮發(fā)性有效成分。本研究擬建立大鼠ig清咽滴丸后血漿中龍腦和薄荷腦同時(shí)測(cè)定的氣相色譜分析方法，闡明清咽滴丸中龍腦、薄荷腦大鼠體內(nèi)藥代動(dòng)力學(xué)特征。方法：血漿樣品采用內(nèi)標(biāo)法并經(jīng)液-液萃取處理，同時(shí)采用氫火焰離子化檢測(cè)器（FID）檢測(cè)龍腦和薄荷腦大鼠體內(nèi)的血藥濃度。結(jié)果：本法可同時(shí)測(cè)定龍腦、薄荷腦的大鼠血漿濃度，并采用DAS（Version 1.0）軟件擬合藥代動(dòng)力學(xué)參數(shù)，結(jié)果表明龍腦和薄荷腦均符合開(kāi)放性一室模型。龍腦在2.5～50.0 ng/μL（r＝0.996 3），薄荷腦在8.7～62.2 ng/μL（r＝0.999 4）均具有良好的線性關(guān)系，最低定量限分別為2.4、5.0 ng/μL。結(jié)論：建立了靈敏、簡(jiǎn)便、準(zhǔn)確的氣相色譜法，該方法適用于清咽滴丸中冰片和薄荷腦體內(nèi)藥代動(dòng)力學(xué)研究，為清咽滴丸制劑的臨床合理用藥提供了理論依據(jù)。

Objective: Borneol and menthol are both main active substances of Qingyan Drop Pills. To establish a method for simultaneous determination of borneol and menthol in plasma of rats after ig administration of Qingyang Drop Pills by gas chromatography. Methods: The blood sample was carried out by liquid-liquid extraction (LLE) with an internal standard solution in naphthalene. Borneol and menthol were determined by means of flame ionization detection (FID). Results: The pharmacokinetic parameters were estimated by a compartmental method using the DAS software program (Version 1.0). The standard curves were linear over a wide concentration rang of 2.5 ? 50.0 ng/μL (r=0.996 3), 8.7 ? 62.2 ng/μL (r=0.999 4) for both borneol and menthol in plasma, respectively. The limit of quantification (LOQ) of borneol and menthol in plasma was 2.4 and 5.0 ng/μL, respectively. Conclusion: This validated assay method has been successfully applied to a pharmacokinetic study of borneol and menthol in plasma of rats after ig administration of Qingyan Drop Pills. The pharmacokinetic parameters provide some information for clinical administration of Qingyan Drop Pills.

國(guó)家自然科學(xué)基金（項(xiàng)目編號(hào)：30772778）、國(guó)家科技支撐計(jì)劃（項(xiàng)目編號(hào)：2007BAI47B04）。