目的：分析胃腸安丸方中的瀉下成分與止瀉成分，并對(duì)具有瀉下作用的大黃蒽醌進(jìn)行定量測(cè)定。方法：利用LC-MS對(duì)胃腸安丸復(fù)方中的主要成分進(jìn)行定性分析。色譜柱：Kromasil C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相：甲醇-0.5％醋酸溶液梯度洗脫；體積流量1.0 mL/min；檢測(cè)波長(zhǎng)254 nm。結(jié)果：蘆薈大黃素、大黃酸、大黃素、大黃酚、大黃素甲醚總質(zhì)量分?jǐn)?shù)在1.03 mg/g以上，平均回收率為100.38％，所得結(jié)果的RSD值均小于2％。結(jié)論：LC-MS分析確定了胃腸安丸中的12個(gè)成分，其中既有止瀉作用成分又有瀉下作用成分，對(duì)其中具有明顯瀉下作用的蒽醌類化合物進(jìn)行定量檢測(cè)，方法簡(jiǎn)便、靈敏、準(zhǔn)確，為探討胃腸安丸的作用物質(zhì)基礎(chǔ)提供數(shù)據(jù)支持。

Objective: To analyze the main components and establish an HPLC method for determination of free anthraquinones content in Weichang’an Pill. Methods: The separation was performed on ODS column (250 mm × 4.6 mm, 5 μm) of Kromasil. The mobile phase was a mixture of methanol-0.5% acetic acid solution, gradient elution, the flow rate was 1.0 mL/min and the wavelength was at 254 nm. Results: Twelve components of Weichang’an Pill were identified, including antidiarrhea and purgative constituents, simultaneously the total content of aloe emodin, rhein, emodin, chrysophanol, and physcion was determined at 1.03 mg/g. The average recovery rate was 100.38%, RSD of the results are all less than 2%. Conclusion: The method is simple, accurate, sensitive, and can be used to provide the data for the quality control of Weichang’an Pill.