目的：建立體外抗癌天然藥物篩選模型，評價丹參素的抗癌活性。方法：以遺傳物質(zhì)DNA為靶標(biāo)，溴化乙錠（EB）為致癌物質(zhì)，丹參素為試藥，利用熒光光譜檢測丹參素對EB-DNA體系的熒光猝滅效應(yīng)；利用圓二色譜技術(shù)檢測丹參素對EB-DNA體系的干擾作用。結(jié)果：丹參素能明顯抑制EB-DNA體系的熒光強度，且具有濃度依賴性；丹參素能夠有效阻斷EB嵌入DNA的堿基對中，維持DNA分子的正常構(gòu)象。結(jié)論：丹參素抑制致癌劑對DNA鏈的嵌合，從而起抗癌活性。該模型可應(yīng)用于抗癌功效的天然藥物的篩選中。

Objective: To establish in vitro screening model for natural anticancer drugs and evaluate the anticancer activity of denshensu on carcinogenic damaged DNA. Methods: Genetic material DNA was used as target, ethidium bromide (EB) as carcinogen, quenching effect of denshensu on fluorescence spectrum of EB-DNA was detected. Interfering effect of denshensu on circular dichroism spectra of EB-DNA was measured. Results: Denshensu strongly quenched the fluorescent intensity at a dose-dependent manner. The circular dichroism spectra showed that denshensu could inhibit the insertion of EB to base pairs of DNA, and maintain the normal conformation of DNA. Conclusion: Denshensu possesses strong anticancer activity by inhibiting insertion of carcinogens into genetic DNA. This model would be applicable for screening natural anticancer drugs

國家自然科學(xué)基金（30873400）; 國家重大新藥創(chuàng)制專項資助（2009ZX09301）。