[關(guān)鍵詞]
[摘要]
目的:建立體外抗癌天然藥物篩選模型,評(píng)價(jià)丹參素的抗癌活性。方法:以遺傳物質(zhì)DNA為靶標(biāo),溴化乙錠(EB)為致癌物質(zhì),丹參素為試藥,利用熒光光譜檢測(cè)丹參素對(duì)EB-DNA體系的熒光猝滅效應(yīng);利用圓二色譜技術(shù)檢測(cè)丹參素對(duì)EB-DNA體系的干擾作用。結(jié)果:丹參素能明顯抑制EB-DNA體系的熒光強(qiáng)度,且具有濃度依賴性;丹參素能夠有效阻斷EB嵌入DNA的堿基對(duì)中,維持DNA分子的正常構(gòu)象。結(jié)論:丹參素抑制致癌劑對(duì)DNA鏈的嵌合,從而起抗癌活性。該模型可應(yīng)用于抗癌功效的天然藥物的篩選中。
[Key word]
[Abstract]
Objective: To establish in vitro screening model for natural anticancer drugs and evaluate the anticancer activity of denshensu on carcinogenic damaged DNA. Methods: Genetic material DNA was used as target, ethidium bromide (EB) as carcinogen, quenching effect of denshensu on fluorescence spectrum of EB-DNA was detected. Interfering effect of denshensu on circular dichroism spectra of EB-DNA was measured. Results: Denshensu strongly quenched the fluorescent intensity at a dose-dependent manner. The circular dichroism spectra showed that denshensu could inhibit the insertion of EB to base pairs of DNA, and maintain the normal conformation of DNA. Conclusion: Denshensu possesses strong anticancer activity by inhibiting insertion of carcinogens into genetic DNA. This model would be applicable for screening natural anticancer drugs
[中圖分類號(hào)]
[基金項(xiàng)目]
國(guó)家自然科學(xué)基金(30873400); 國(guó)家重大新藥創(chuàng)制專項(xiàng)資助(2009ZX09301)。