目的：建立測(cè)定血漿中委陵菜黃酮的分析方法，初步對(duì)該化合物在大鼠體內(nèi)的經(jīng)時(shí)過(guò)程進(jìn)行考察。方法：建立委陵菜黃酮的HPLC測(cè)定方法，考察大鼠iv委陵菜黃酮（10、20、40 mg/kg）后血藥濃度-時(shí)間曲線。結(jié)果：大鼠尾iv 3 個(gè)劑量委陵菜黃酮后，血漿藥時(shí)曲線符合三室模型，藥時(shí)曲線下面積AUC_0-180分別為346.05、455.74和1 655.14 μg/（mL·min），分布相半衰期t_1/2α分別為103.90、15.59和9.49 min，消除相半衰期t_1/2β分別為30 580.98、2 045.70和894.84 min。結(jié)論：iv給藥后，委陵菜黃酮血漿藥時(shí)曲線符合三室模型，在大鼠體內(nèi)迅速分布，但消除速率較慢。

Objective: To establish the method of measuring concentration of flavones from Potentilla chinensis in plasma of rats and to explore its pharmacokinetic features. Methods: An HPLC analysis method for the determination of flavones in P. chinensis was established and used to analyze the plasma concentration-time curve on iv injection of flavones in P. chinensis to rats. Results: A RP-HPLC analysis method with C-18 column for the determination of flavones in P. chinensis was established. After the injection of 10, 20, and 40 mg/kg of flavones in P. chinensis, the plasma concentration-time curve was in line with the three-compartment model, AUC0-180 were 346.05, 455.74, and 1 655.14 μg/(mL·min), respectively. Distribution phase half-life t_1/2α was 103.90, 15.59, and 9.49 min, and elimination phase half-life t_1/2β were 30 580.98, 2 045.70, and 894.84 min, respectively. Conclusion: After iv administration, the plasma concentration-time curve of flavones in P. chinensis is in line with the three-compartment model, the rapid distribution, while the slower rate of elimination in rats.

教育部博士點(diǎn)基金項(xiàng)目(200800620007)