目的：建立血府逐瘀膠囊原料藥材紅花中羥基紅花黃色素A和蘆丁，川芎中生物堿類(lèi)成分川芎嗪和有機(jī)酸類(lèi)物質(zhì)阿魏酸的定量的測(cè)定方法，從而建立多成分的定量標(biāo)準(zhǔn)。方法：采用HPLC法，色譜條件（紅花）：C₁₈色譜柱，以甲醇-0.4%磷酸水溶液為流動(dòng)相進(jìn)行梯度洗脫，體積流量 0.5 mL/min，柱溫 40 ℃。色譜條件（川芎）：C₁₈色譜柱，以甲醇-1%冰醋酸水溶液（32︰68）為流動(dòng)相，體積流量 1.0 mL/min，柱溫30 ℃。結(jié)果： 平均回收率分別為羥基紅花黃色素A 97.2%、RSD為2.3%（n＝6）；蘆丁99.6%、RSD為3.2%（n＝6）；川芎嗪100.9%、RSD為2.2%（n＝6）阿魏酸98.6%、RSD為2.6%（n＝6）。結(jié)論：本方法簡(jiǎn)便、快速、準(zhǔn)確，可作為血府逐瘀膠囊的原料藥材的質(zhì)量控制方法。

Objective: To establish the HPLC methods for the determination of hydroxysafflor yellow A and rutin in raw material and ligustrazine and ferulic acid in Ligusticum chuanxiong of Xuefuzhuyu Capsula and quantitative criteria for multi-component. Methods: The HPLC was applied with a C₁₈ column for the determination of Carthamus tinctorius by gradient elution using methanol -0.4% phosphonic acid solution as the mobile phase. The flow rate was 0.5 mL/min and the temperature of column was 40?C. The HPLC was applied with a C₁₈ column for the determination of L. chuanxiong using methanol -1.0% acetic acid solution (32︰68) as the mobile phase. The flow rate was 1.0 mL/min and the temperature of column was 30?C. Results: The average recovery rates were 97.2% ,99.6% ,100.9% , and 98.6%, RSD were 2.3%, 3.2%, 2.2%, and 2.6% for,hydroxysafflor yellow A and rutin, ligustrazine and ferulaic acid, respectively (n=6). Conclusion: The method is simple ,rapid, and accurate. It can be used for the quality control of Xuefuzhuyu Capsula.

天津市科技創(chuàng)新專項(xiàng)資金項(xiàng)目（06FZZDSH00404）