目的：建立當歸的HPLC指紋圖譜，并對其化學成分進行分析。方法：采用高效液相色譜法，以甲醇-0.05％磷酸水溶液系統(tǒng)進行線性梯度洗脫，測定了來源于岷縣的多批當歸藥材和市售當歸飲片的指紋圖譜，應(yīng)用相似度分析和聚類分析進行分類；并采用液相色譜-質(zhì)譜聯(lián)用（HPLC-MS）方法對岷縣當歸藥材中的化學成分進行定性分析。結(jié)果：在選定的色譜條件下，得到當歸HPLC指紋圖譜，標定出12個共有峰；根據(jù)相似度分析和聚類分析的結(jié)果，將當歸藥材和當歸飲片各分為1類。HPLC-MS鑒定出阿魏酸，6,7-二羥基藁本內(nèi)酯，洋川芎內(nèi)酯I、H、A，阿魏酸松柏酯，E-藁本內(nèi)酯，Z-藁本內(nèi)酯，3-正丁烯基苯肽共9個化學成分。結(jié)論：應(yīng)用本方法評價當歸的質(zhì)量是可行的。

Objective: To establish the HPLC fingerprint of Angelicae Sinensis Radix and analyze the chemical components by HPLC-MS. Methods: The HPLC method was used with Accurasil-C₁₈ column (250 mm × 4.6 mm, 5 μm), and methanol -0.05% phosphoric acid was used as mobile phase in a gradient elution. The HPLC fingerprint was established for the determination of several batches of Angelicae Sinensis Radix grown in Minxian and praeparata Angelicae Sinensis Radix sold in the market and the similarity, and cluster analysi were carried out. The chemical components of Angelicae Sinensis Radix grown in Minxian were analysed by HPLC-MS. Results: Under the selected spectrum condition, 12 public peaks were shown in the HPLC fingerprint. Angelicae Sinensis Radix was classified into two groups based on the result of similarity and cluster analysi including Angelicae Sinensis Radix grown in Minxian and praeparata Angelicae Sinensis Radix sold in the market. Ferulic acid, 6,7-dihydroxydihydroligustilide, senkyunolide I, senkyunolide H, senkyunolide A, coniferyl ferulate, E-ligustilide, Z-ligustilide, and 3-butylidenephthalide were identified in chromatograms based on their MS data. Conclusion: This method is reasonable and reliable to the quality control of Angelicae Sinensis Radix.

“十一五” 國家科技支撐計劃課題（2007BAI37B06）。