18 column (250 mm × 4.6 mm, 5 μm), and methanol -0.05% phosphoric acid was used as mobile phase in a gradient elution. The HPLC fingerprint was established for the determination of several batches of Angelicae Sinensis Radix grown in Minxian and praeparata Angelicae Sinensis Radix sold in the market and the similarity, and cluster analysi were carried out. The chemical components of Angelicae Sinensis Radix grown in Minxian were analysed by HPLC-MS. Results: Under the selected spectrum condition, 12 public peaks were shown in the HPLC fingerprint. Angelicae Sinensis Radix was classified into two groups based on the result of similarity and cluster analysi including Angelicae Sinensis Radix grown in Minxian and praeparata Angelicae Sinensis Radix sold in the market. Ferulic acid, 6,7-dihydroxydihydroligustilide, senkyunolide I, senkyunolide H, senkyunolide A, coniferyl ferulate, E-ligustilide, Z-ligustilide, and 3-butylidenephthalide were identified in chromatograms based on their MS data. Conclusion: This method is reasonable and reliable to the quality control of Angelicae Sinensis Radix."/>