目的：從牛蒡子中分離純化出有效成分，并對(duì)其分子結(jié)構(gòu)進(jìn)行鑒定與表征。方法：采用醇提酸解法提取，分離出粗品，經(jīng)過對(duì)粗品進(jìn)行脫脂、結(jié)晶處理，得到無色棱狀晶體。采用紫外光譜（UV）、紅外光譜（FTIR）、質(zhì)譜（MS）、氫譜（H-NMR）、碳譜（C-NMR）及單晶衍射對(duì)該有效成分進(jìn)行組成和分子結(jié)構(gòu)的鑒定與表征。結(jié)果：從牛蒡子粗粉中經(jīng)提取分離，得到結(jié)晶，提取率為4.46％，顯色反應(yīng)及波譜分析均顯示為木脂素類化合物。結(jié)論：該有效成分鑒定為牛蒡子苷元，即（3R,4R）-4-[(3,4-二甲氧基苯基)甲基]二氫-3-[(4-羥基-3-甲氧基苯基)甲基]-2（3H）-呋喃酮，分子式為C₂₁H₂₄O₆。

Objective: To isolate and purify active component from Arctii Fructus and identify and characterize. Methods: The crude product was separated from Arctii Fructus by alcohol extraction and acid hydrolysis. Using recrystallization its structure was method, colorless prismatic crystal was obtained. The composition and molecular structure were characterized by UV, FTIR, MS, ¹H-NMR, ¹³C-NMR, and X-ray analyses. Results: The extracting rate of the crystal gained from the powder of Arctii Fructus was 4.46%. Both color reactions and spectral analyses indicated that the compound was lignan. Conclusion: The active component is characterized as (3R, 4R)-4-[(3,4-dimethoxyphenyl) methyl] dihydro-3-[(4-hydroxy-3-methoxy phenyl) methyl]-2(3H)-furanone (arctigenin, ARC-G) and the molecular formula is C₂₁H₂₄O₆.

“十一五”國家科技支撐計(jì)劃資助項(xiàng)目（2007BAI41B06）。