介紹應(yīng)用熒光定量PCR技術(shù)對基因治療藥物組織分布研究的實驗方法。從以下幾個方面，即定量PCR技術(shù)原理、實驗設(shè)計思路、各種檢測方法的比較來說明這一問題。其中，可以使用的檢測方法包括紫外定量法和引入內(nèi)參基因法，后者又可以分為外標(biāo)法和內(nèi)標(biāo)法。充分對比了這些方法的優(yōu)缺點，并結(jié)合本實驗室的研究現(xiàn)狀，介紹了本監(jiān)測中心在選用外標(biāo)法進行研究時所取得的一些成果和實驗經(jīng)驗。

The paper focuses on the test methodology of applying real-time PCR to gene therapy drug biodistribution research. It is illuminated from three aspects containing real-time PCR elements, examination design and various methods comparing. Test results can be detected by ultraviolet and internal control gene method composing of external standard and internal reference techniques. The advantage and deficiency of these methods are fully discussed in this paper, as well as some successful and experimental experiences are reported in the research using external standard method.