介紹應(yīng)用熒光定量PCR技術(shù)對基因治療藥物組織分布研究的實(shí)驗(yàn)方法。從以下幾個(gè)方面，即定量PCR技術(shù)原理、實(shí)驗(yàn)設(shè)計(jì)思路、各種檢測方法的比較來說明這一問題。其中，可以使用的檢測方法包括紫外定量法和引入內(nèi)參基因法，后者又可以分為外標(biāo)法和內(nèi)標(biāo)法。充分對比了這些方法的優(yōu)缺點(diǎn)，并結(jié)合本實(shí)驗(yàn)室的研究現(xiàn)狀，介紹了本監(jiān)測中心在選用外標(biāo)法進(jìn)行研究時(shí)所取得的一些成果和實(shí)驗(yàn)經(jīng)驗(yàn)。

The paper focuses on the test methodology of applying real-time PCR to gene therapy drug biodistribution research. It is illuminated from three aspects containing real-time PCR elements, examination design and various methods comparing. Test results can be detected by ultraviolet and internal control gene method composing of external standard and internal reference techniques. The advantage and deficiency of these methods are fully discussed in this paper, as well as some successful and experimental experiences are reported in the research using external standard method.