目的：建立川續(xù)斷的HPLC指紋圖譜分析方法。方法：采用高效液相色譜技術(shù)，Hypersil-C₁₈色譜柱（250 mm ×4.6 mm, 5 μm）；流動相為乙睛-水，梯度洗脫；體積流量1.0 mL/min；檢測波長212 nm；進(jìn)樣量30 μL；柱溫 30 ℃。結(jié)果：建立了以 15 個共有峰為特征指紋信息的HPLC指紋圖譜，發(fā)現(xiàn)各產(chǎn)地和來源的川續(xù)斷的指紋圖譜有一定的差異。結(jié)論：該指紋圖譜方法準(zhǔn)確可靠，重現(xiàn)性好，可作為川續(xù)斷內(nèi)在質(zhì)量評價的依據(jù)。

Objective: To establish the HPLC fingerprints of Dipsacus asperoides processed with methanol. Methods: HPLC analysis was performed on a Hypersil-C₁₈ column (250 mm × 4. 6 mm, 5 μm) with mobile phase: acetonitrile-water under isocratic and gradient elutions and detection wavelength was 212 nm; The flow rate was 1.0 mL/min and column temperature was 30 ℃. Results: HPLC fingerprint by taking 15 common peaks as characteristic information was establised. It was found that the preparative HPLC of D. asperoides from different habitats and sources has some differences. Conclusion: The method is reliable and accurate, and can be used for the quality control of D. asperoides.

科技部國際合作項目(2008DFB30070)；天津市重點項目(06TXTJJCB600)