雙氧水處理后造成CHL細(xì)胞產(chǎn)生明顯的DNA損傷，呈現(xiàn)不同彗尾長(zhǎng)度的彗星，與陰性對(duì)照組比較有顯著差異。不同質(zhì)量濃度的地黃提取物處理細(xì)胞后，彗星長(zhǎng)、彗尾長(zhǎng)度等各項(xiàng)指標(biāo)與陰性對(duì)照組比較無(wú)顯著差異。結(jié)論 單細(xì)胞凝膠電泳檢測(cè)DNA損傷的敏感度較高，不同質(zhì)量濃度的地黃提取物對(duì)CHL細(xì)胞未產(chǎn)生DNA損傷。;Objective To study the DNA damage of Chinese hamster lung (CHL) fibroblas cell line induced by Rehmanniae Radix extract using single cell gel electrophoresis (SCGE) (comet assay) so as to provide the reference for preclinical safety evaluation of the drug. Methods Taking H2O2 as positive drug, the cells were treated with Rehmanniae Radix extract at the concentrations of 0.2, 1.0, and 5.0 mg/mL, respectively for 24 h, then the comet assays were performed. Results The obvious damage of CHL cells was induced by H2O2 and the significant difference was indicated by the the average tail length of comet in compared with the negative control group. There was no significant difference of every index, such as comet length and comet tail length at various concentrations of Rehmanniae Radix extract. Conclusion The comet assay shows considerable sensitivity to detect the lesion process. Different concentrations of Rehmanniae Radix extract could not induce DNA damage to CHL cells."/> 2O2"/>

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首頁(yè) > 過(guò)刊瀏覽>2011年第34卷第1期 >2011,34(1):26-28. DOI:10.7501/j.issn.0253-6376.[year].1.[sequence]
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單細(xì)胞凝膠電泳分析地黃提取物對(duì)倉(cāng)鼠肺成纖維細(xì)胞DNA的損傷作用

Experimental study of long-term toxicity comparative test between spironolactone sample group and control group in rats

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    [關(guān)鍵詞]
    [摘要]
    目的 采用單細(xì)胞凝膠電泳（彗星實(shí)驗(yàn)）技術(shù)研究地黃提取物致倉(cāng)鼠肺成纖維細(xì)胞（CHL細(xì)胞）DNA的損傷作用，為該藥物臨床前安全性評(píng)價(jià)提供參考。方法 采用0.2、1.0、5.0 mg/mL地黃提取物，用雙氧水作為陽(yáng)性對(duì)照藥，處理細(xì)胞，24 h后收獲細(xì)胞，進(jìn)行彗星電泳實(shí)驗(yàn)。結(jié)果/b> 雙氧水處理后造成CHL細(xì)胞產(chǎn)生明顯的DNA損傷，呈現(xiàn)不同彗尾長(zhǎng)度的彗星，與陰性對(duì)照組比較有顯著差異。不同質(zhì)量濃度的地黃提取物處理細(xì)胞后，彗星長(zhǎng)、彗尾長(zhǎng)度等各項(xiàng)指標(biāo)與陰性對(duì)照組比較無(wú)顯著差異。結(jié)論 單細(xì)胞凝膠電泳檢測(cè)DNA損傷的敏感度較高，不同質(zhì)量濃度的地黃提取物對(duì)CHL細(xì)胞未產(chǎn)生DNA損傷。
    [Key word]
    [Abstract]
    Objective To study the DNA damage of Chinese hamster lung (CHL) fibroblas cell line induced by Rehmanniae Radix extract using single cell gel electrophoresis (SCGE) (comet assay) so as to provide the reference for preclinical safety evaluation of the drug. Methods Taking H2O2 as positive drug, the cells were treated with Rehmanniae Radix extract at the concentrations of 0.2, 1.0, and 5.0 mg/mL, respectively for 24 h, then the comet assays were performed. Results The obvious damage of CHL cells was induced by H2O2 and the significant difference was indicated by the the average tail length of comet in compared with the negative control group. There was no significant difference of every index, such as comet length and comet tail length at various concentrations of Rehmanniae Radix extract. Conclusion The comet assay shows considerable sensitivity to detect the lesion process. Different concentrations of Rehmanniae Radix extract could not induce DNA damage to CHL cells.
    [中圖分類(lèi)號(hào)]
    [基金項(xiàng)目]
    基金項(xiàng)目：“十一五”國(guó)家科技支撐計(jì)劃課題（2007BAI37B06）
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