目的 研究片仔癀對體外腫瘤細胞增殖的影響，以及對雞胚尿囊膜血管生成的抑制作用。方法 采用MTT法檢測片仔癀對體外培養(yǎng)腫瘤細胞（肝癌SMMC-7721細胞、胃癌SGC-7901細胞、宮頸癌HeLa細胞）的抗腫瘤作用；以雞胚尿囊膜血管生成（CAM）實驗，研究片仔癀對新生血管生成的抑制作用。結(jié)果 片仔癀在0.2～1.0 mg/mL對各腫瘤細胞的增殖有顯著抑制作用，1.0 mg/mL片仔癀對肝癌細胞增殖抑制率達到81.55%；片仔癀在0.1～1 000 μg/mL對雞胚尿囊膜血管生成有顯著抑制作用，給藥雞胚的新生血管數(shù)減少，血管變細，其中10 μg/mL抑制率最高，一級血管抑制率為61.1%，二級血管抑制率為59.1%。結(jié)論 在實驗給藥濃度下，片仔癀對受試腫瘤細胞的增殖具有較強抑制作用，對雞胚尿囊膜血管生成有顯著抑制作用。

_Objective To study the effect of Pien Tze Huang (PTH) on tumor proliferation in vitro and chorioallantoic membrane (CAM) angiogenesis. _Methods The effect of PTH on the proliferation of cancer cells (SMMC-7721, SGC-7901, and HeLa) were evaluated by MTT assay. The CAM angiogenesis model was established to determine the inhibitive effect of PTH against angiogenesis. _Results PTH had significant inhibitive effect on tumor cells proliferation at doses of 0.2—1.0 mg/mL. With the dose of 1.0 mg/mL, the inhibition ratio of PTH was 81.55% on hepatoma carcinoma cell SMMC-7721; PTH significantly affected chick embryonic angiogenesis in doses range of 0.1—1 000 μg/mL and induced a reduction in the capillary plexus of the CAM. The central and secondary capillaries were reduced by 61.1% and 59.1% respectively, at the dose of 10 μg/mL. _Conclusion PTH showed significant inhibition effect on tumor proliferation and chorioallantoic membrane angiogenesis in the doses of this study.

國家支撐計劃項目（2007BAI36B01），江蘇省“六大人才高峰”資助項目（第五批次）