目的 通過對HPLC-DAD-MS多種分析手段的聯(lián)用，建立大黃藥材成分分析方法，確認(rèn)各個特征峰的歸屬，從而定性定量的評價藥材質(zhì)量，預(yù)測不同道地產(chǎn)區(qū)大黃藥材的藥效，為譜效學(xué)研究提供基礎(chǔ)研究。方法 以全國各道地產(chǎn)區(qū)大黃藥材為研究對象，采用Agilent Zorbox C₁₈（250 mm×4.6 mm，5 μm）色譜柱，乙睛–0.1%磷酸水二元梯度洗脫模式，體積流量為1.0 mL/min，檢測波長280 nm，建立了20批來自四川、青海、甘肅地區(qū)的大黃藥材的HPLC圖譜，并通過HPLC-DAD-MS聯(lián)用技術(shù)對其中30個特征峰進(jìn)行了歸屬分類，同時進(jìn)行了各類指紋成分比較研究。結(jié)果 各地區(qū)間游離蒽醌量差異較小，而其他成分差異明顯，表現(xiàn)出可能存在的質(zhì)量差異。結(jié)論 本實驗全面闡明了大黃藥材的各主要化學(xué)成分及地區(qū)間差異，方法樣品處理簡單，建立的大黃分析方法穩(wěn)定性、重復(fù)性好，為大黃藥材的質(zhì)量評價提供較為充實的參考依據(jù)。

_Objective To establish a stable chromatography method of ingredient analysis in the traditional Chinese medicinal materials (TCMM) Rhei Radix et Rhizoma using HPLC-DAD-MS,and identify the attribution of chromatographic peaks. By the chromatography method, the quality of TCMM Rhei Radix et Rhizoma was evaluated by the perspectives of both quality and quantity, and the medicinal effect of Rhei Radix et Rhizoma from different sources was predicted. This study could also provide thoughts and research foundation for chromatographic pharmacodynamics. _Methods Agilent Zorbox C₁₈ column (250mm × 4.6 mm, 5 μm), with mixture of acetonitrile and 0.1% phosphoric acid as mobile phase, flow rate 1.0 mL/min, detective wavelength 280 nm, was used to acquire HPLC chromatogram of 20 Rhei Radix et Rhizoma from different sources in Sichuan, Qinghai, and Gansu provinces. The 30 characteristic peaks were identified and classified using HPLC-DAD-MS and the quantity each various ingredients were compared in fingerprint in this study. _Results The results showed that the difference of anthraquinone content from different intervals was not significant, but the significant differences existed in other components, which indicated the quality difference might exist. _Conclusion With the simple sample treatment technique, the method of Rhei Radix et Rhizoma established is stable and repeatable, which provides a solid reference for the quality control of Rhei Radix et Rhizoma.