目的 建立九香止瀉片中總鞣質(zhì)的定量測(cè)定方法。方法 用干酪素法測(cè)定九香止瀉片中總鞣質(zhì)。測(cè)定條件為：以水超聲提取30 min，干酪素用量800 mg，29%碳酸鈉溶液顯色30 min后測(cè)定。結(jié)果 線性回歸方程為A＝102.65 C＋0.026 3，r＝0.999 5，鞣酸在1.0～10.0 μg/mL線性關(guān)系良好，平均回收率為96.58%，RSD值為0.615%（n＝6）。結(jié)論 該分析方法簡(jiǎn)便、準(zhǔn)確、可靠，可用于測(cè)定九香止瀉片中總鞣質(zhì)。

Objective To establish a stable method for determination of total tannins in Jiuxiang Zhixie Tablets. Methods The casein method was used to determine the content of total tannins of Jiuxiang Zhixie Tablets. The conditions of determination is: Supersonic withdraws 30 min by water, used casein 800 mg, coloration in 29% the Na₂CO₃ 30 min. Results The linearity range was 1.0—10.0 μg/mL, r = 0.999 5. The average recovery was 96.58% with RSD of 0.615% (n = 6). Conclusion The method is reliable and could be used to determine the total tannins of Jiuxiang Zhixie Tablets.

湖南省教育廳重點(diǎn)項(xiàng)目（05A0312）