目的 建立冠脈通片的質(zhì)量控制方法。方法 采用TLC法，分別以甲苯-醋酸乙酯-甲酸（15∶2∶1）、乙酸丁酯-甲酸-水（1.3∶1∶1）10 ℃下放置的上層溶液為展開(kāi)劑對(duì)何首烏及淫羊藿進(jìn)行薄層色譜鑒別；采用氣相色譜法，以水楊酸甲酯為內(nèi)標(biāo)物，安捷倫DB-WAX毛細(xì)管柱（30 m×0.53 mm，1 μm），F(xiàn)ID檢測(cè)器，載氣為氮?dú)?，柱溫?40 ℃，測(cè)定冠脈通片中的冰片；采用高效液相色譜法，Dikma-C₁₈色譜柱（250 mm×4.6 mm，5 μm），甲醇-0.5%乙酸（88∶12）為流動(dòng)相，體積流量1.0 mL/min，檢測(cè)波長(zhǎng)281 nm，柱溫25 ℃，測(cè)定冠脈通片中的丹參素。結(jié)果 薄層鑒別中樣品色譜斑點(diǎn)清晰，分離較好；氣相色譜中，冰片中龍腦及內(nèi)標(biāo)物水楊酸甲酯均得到良好的分離，冰片的平均回收率為96.72%（RSD為0.67%）；HPLC測(cè)定丹參素在0.100 8～0.604 8 μg與峰面積值線性關(guān)系良好（r＝0.999 8），平均回收率為100.22%（RSD為1.15%）。結(jié)論 本實(shí)驗(yàn)建立的方法簡(jiǎn)便、準(zhǔn)確、可靠，可用于冠脈通片的質(zhì)量控制。

Objective To establish quality standard for Guanmaitong Tablets. Methods Taking methylbenzene-ethyl acetate-formic acid and butyl acetate-formic acid-water as developing agent, Polygoni Multiflori Radix and Epimedii Folium were identified by TLC. The internal standard method was employed. Methyl salicylate, as internal standard, was added to the sample before treatment. The GC system consisted of Agilent DB-WAX, FID as the detector, nitrogen as carrier gas, and column temperature at 140 ℃. Borneol in Guanmaitong Tablets was determined by GC and salvianolic acid A by HPLC. The chromatographic separation was achieved on a Dikma-C₁₈ column with a mobile phase of methanol-0.5% acetic acid solution (88 : 12). The flow rate was 1.0 mL/min; The detection wavelength was set at 281 nm, and column temperature at 25 ℃. Results TLC spots developed were fairly clear and the blank test showed no interference. Camphol borneol and methyl salicylate were separated well under the chromatographic condition. The average recovery of synthetic borneol was 96.72%. The calibration curve was linear within range of 0.100 8—0.604 8 μg (r = 0.999 8). The average recovery was 100.22%. Conclusion The method is accurate, sensitive, and specific. It could be used for the quality control of Guanmaitong Tablets.