目的 研究紅芪總多糖（THPS）單用及與環(huán)磷酰胺（CTX）合用對荷瘤小鼠的抑瘤作用，并探討其機制。方法 昆明種小鼠90只，隨機分10只為正常對照組，其余80只小鼠接種S180瘤株后隨機分為荷瘤對照組、CTX組、THPS高、中、低劑量 [400、200、100 mg/(kg·d)] 組及各與CTX合用組共8組。正常對照組和荷瘤對照組給予生理鹽水，其余各組給予相應劑量THPS及CTX治療，觀察腫瘤的體積變化。14 d后處死小鼠，觀察瘤質量、抑瘤率；血球計數(shù)儀檢測外周血白細胞、血小板、紅細胞及血紅蛋白；流式細胞儀檢測CD3⁺ T細胞和NK細胞。結果 與荷瘤對照組相比，中劑量THPS明顯抑制小鼠S180瘤質量增長（P＜0.01）及體積增加（P＜0.05），提高NK細胞水平（P＜0.05）。與CTX組相比，THPS與CTX合用后，各劑量組均能明顯抑制CTX所致的白細胞數(shù)量下降（P＜0.01）；而只有中劑量的THPS與CTX合用能夠降低腫瘤的質量（P＜0.05），并能明顯抑制CTX所致的CD3⁺T細胞（P＜0.01）和NK細胞（P＜0.05）數(shù)量下降。結論 中劑量的THPS能抑制S180瘤生長，具有降低CTX免疫抑制和骨髓抑制的作用，其機制與提高T細胞和NK細胞介導的免疫應答有關。

Objective To investigate the antitumor effects of total hedysarum polybotrys saccharide (THPS) alone and THPS combinated with cytoxan (CTX) on tumor-bearing mice and its mechanisms. Methods Ninety Kunming mice were divided into nine groups randomly. Except for normal group, other eight groups inoculated with S180 tumor cells consisted of control, CTX, THPS of high, moderate, and low doses, and THPS of high, moderate, and low doses combined with cytoxan groups. Normal and control groups were treated with normal saline while others were treated with CTX, THPS, and THPS combination with CTX. Tumor sizes were measured every other day. All mice were killed after treatment for 14 d. Tumor weight and inhibition rate were observed. CD3⁺T lymphocyte and NK cells were counted through the flow cytometer. Blood routine was tested by blood cell count instrument. Results Compared with the control group, the moderate dose of THPS showed significantly antitumor effect on S180 tumor weight (P<0.01) and size (P<0.05) and markedly increased NK cell amount (P<0.05). Compared with the CTX group, all dose groups of THPS combined with CTX obviously inhibited the decrease of white blood cell amount (P<0.01). Only the moderate dose of THPS combined with CTX significantly reduced S180 tumor weight (P<0.05) and inhibited the decreases of CD3⁺ T lymphocyte (P<0.01) and NK cell (P<0.05) amounts caused by CTX. Conclusion The moderate dose of THPS showed significant antitumor effect in S180-bearing mice and reduced immunosuppression and myelosuppression caused by CTX through improving immune response mediated by T cells and NK cells.

甘肅省科技攻關項目（No.2GS064-A43-020-32）；蘭州大學醫(yī)學科研基金（No.LZUYX200601）