目的 應(yīng)用自動采樣技術(shù)和傳統(tǒng)采樣方法對梓醇在大鼠體內(nèi)的藥動學(xué)進行研究，比較兩種采樣方法對梓醇藥動學(xué)參數(shù)的影響。方法 成年Wistar大鼠12只，隨機分為2組，ig給予大鼠100 mg/kg梓醇供試品后不同時間點分別采用自動采樣技術(shù)與傳統(tǒng)手動采樣方法收集大鼠血樣，經(jīng)LC-MS/MS方法分析測得不同時間的血藥濃度，應(yīng)用DAS 2.0計算程序、非隔室模型計算藥動學(xué)參數(shù)并以單因素方差分析法對兩種方法所得的藥動學(xué)參數(shù)進行評價。結(jié)果 大鼠ig給予梓醇供試品100 mg/kg后，兩組試驗的血藥濃度數(shù)據(jù)的變化趨勢高度一致，兩條平均藥–時曲線趨勢接近，達峰時間分別為1.5、1.0 h，C_max相差10%左右，計算所得各藥動學(xué)參數(shù)單因素方差分析結(jié)果顯示兩組的各個參數(shù)均無顯著差異。結(jié)論 采用兩種方法收集的血樣分析計算所得的梓醇在大鼠體內(nèi)藥動學(xué)特征基本相同。

Objective To study the pharmacokinetics of catalpol in rats by two different blood sampling techniques (manual sampling vs automated sampling) and compare the two methods on pharmacokinetics of catalpol. Methods Two different blood sampling techniques were used in two groups of rats after ig administration of 100 mg/kg catalpol. The plasma concentration of catalpol was determinated by LC-MS/MS method. The main pharmacokinetic parameters were obtained by DAS 2.0 program and evaluated by Oneway ANOVA. Results After ig administration of 100 mg/kg catalpol to the rats, the concentration-time curves of both sampling techniques were similar, and the t_max were 1.5 and 1.0 h. C_max of automated sampling group was 10% higher than that of the manual sampling group. Conclution The pharmacokicetic parameters of two groups are basically the same.

國家重點基礎(chǔ)研究發(fā)展計劃項目（2010CB735602）