[關鍵詞]
[摘要]
目的 觀察參丹芎注射液是否具有血管刺激性、溶血和過敏性反應。方法 家兔耳緣iv給予參丹芎注射液8 mL/kg,每天給藥1次,連續(xù)給藥5 d,停藥48 h后對注射部位進行病理組織學檢查;0.1~0.5 mL參丹芎注射液在4.5~4.9 mL兔紅細胞混懸液中放置0.25~3 h,觀察對兔紅細胞懸液的溶血及有無紅細胞凝聚作用;豚鼠隔日ip給予0.5 mL參丹芎注射液,連續(xù)注射3次,分別于末次致敏后第14和21天iv給予1.0 mL參丹芎注射液進行激發(fā),觀察豚鼠30 min內是否出現過敏反應。結果 參丹芎注射液對家兔耳緣靜脈血管內皮沒有損傷和刺激作用;兔紅細胞溶血和凝聚作用陰性;豚鼠全身主動過敏反應陰性。結論 參丹芎注射液無血管刺激性、體外溶血性和全身主動過敏性反應。
[Key word]
[Abstract]
Objective To observe whether Shendanxiong Injection (SI) has vascular stimulation, hemolyzation, and sensitization. Methods Rabbits were iv administrated with SI (8 mL/kg, once daily continuously for 5 d), and histopathlogic examination of injection site was carried out after 48 h of drug withdrawal. SI (0.1—0.5 mL) was placed in rabbit erythrocyte (4.5—4.9 mL) in vitro for 0.25—3 h, and hemolyzation and agglutination of rabbit erythrocyte were observed. Guinea pigs were ip administrated with SI (0.5 mL) every other day for three times successively, and on Day 14 and 21 after the last sensitization 1.0 mL of SI was iv administrated to arouse the reaction. Results It showed that SI had no vascular irritation or injury effect on rabbit vascular endothelial cells, and no evidence of hemolyzation and aggregation on rabbit erythrocyte in vitro or allergic reaction on guinea pig in vivo were observed. Conclusion There is no vascular stimulation on rabbit vascular endothelial cells, no sensitization on guinea pig in vivo, or no hemolyzation oragglutination on rabbit red blood cells in vitro.
[中圖分類號]
[基金項目]
國家創(chuàng)新藥物資助項目(2009ZX09103-445)