18柱（250 mm×4.6 mm，5 μm）；流動(dòng)相為甲醇-0.2%磷酸溶液，線性梯度洗脫，體積流量為1.0 mL/min，檢測(cè)波長(zhǎng)為324、280 nm，柱溫30 ℃。結(jié)果 綠原酸、連翹苷、黃芩苷的線性范圍分別為0.093～1.860、0.022～0.440、0.328～6.560 μg，平均加樣回收率分別為98.8%、98.4%、99.3%。結(jié)論 該方法簡(jiǎn)便、快速、準(zhǔn)確，可作為雙黃連制劑的質(zhì)量控制方法。;Objective To establish an HPLC method for simultaneously determining chlorogenic acid, forsythin, and baicalin in Shuanghuanglian preparations. Methods HPLC assay was carried out using an Agilent ZORBAX XDB C18 column with column temperature at 30℃; The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution with flowing rate of 1.0 mL/min; And the detection wavelengths were 324 and 280 nm. Results The linear ranges of chlorogenic acid, forsythin, and baicalin were 0.093—1.860, 0.022—0.440, and 0.328—6.560 μg respectively, and the average recoveries of three components were 98.8%, 98.4%, and 99.3% respectively. Conclusion The method is simple, quick, and accurate, and may be used for the quality control of Shuanghuanglian preparations."/>

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首頁 > 過刊瀏覽>2012年第35卷第2期 >2012,35(2):113-116. DOI:10.7501/j.issn.0253-6376.[year].2.[sequence]
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HPLC法同時(shí)測(cè)定雙黃連制劑中綠原酸、連翹苷、黃芩苷

Simultaneous determination of chlorogenic acid, forsythin, and baicalin in Shuanghuanglian preparations by dual-wavelength HPLC

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    [關(guān)鍵詞]
    [摘要]
    目的 建立同時(shí)測(cè)定雙黃連制劑中綠原酸、連翹苷和黃芩苷的HPLC方法。方法 色譜柱為Agilent ZORBAX XDB C18柱（250 mm×4.6 mm，5 μm）；流動(dòng)相為甲醇-0.2%磷酸溶液，線性梯度洗脫，體積流量為1.0 mL/min，檢測(cè)波長(zhǎng)為324、280 nm，柱溫30 ℃。結(jié)果 綠原酸、連翹苷、黃芩苷的線性范圍分別為0.093～1.860、0.022～0.440、0.328～6.560 μg，平均加樣回收率分別為98.8%、98.4%、99.3%。結(jié)論 該方法簡(jiǎn)便、快速、準(zhǔn)確，可作為雙黃連制劑的質(zhì)量控制方法。
    [Key word]
    [Abstract]
    Objective To establish an HPLC method for simultaneously determining chlorogenic acid, forsythin, and baicalin in Shuanghuanglian preparations. Methods HPLC assay was carried out using an Agilent ZORBAX XDB C18 column with column temperature at 30℃; The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution with flowing rate of 1.0 mL/min; And the detection wavelengths were 324 and 280 nm. Results The linear ranges of chlorogenic acid, forsythin, and baicalin were 0.093—1.860, 0.022—0.440, and 0.328—6.560 μg respectively, and the average recoveries of three components were 98.8%, 98.4%, and 99.3% respectively. Conclusion The method is simple, quick, and accurate, and may be used for the quality control of Shuanghuanglian preparations.
    [中圖分類號(hào)]
    [基金項(xiàng)目]
    江西科技師范學(xué)院重點(diǎn)科研項(xiàng)目（KY2009ZZ04）
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