目的 建立產(chǎn)后大鼠子宮炎癥模型，從分子生物學角度探討益母草對產(chǎn)后子宮復舊的內(nèi)在機制。方法 將大鼠隨機分為4組：空白對照組、模型組、益母草組、三七總皂苷組。建立產(chǎn)后子宮炎癥模型，用三七總皂苷作陽性對照，采用ELISA法對各組大鼠血清腫瘤壞死因子-α（TNF-α）的量進行測定，對各組大鼠的子宮組織進行HE染色觀察，同時用免疫組化法檢測子宮組織中基質金屬蛋白酶-13（MMP-13）、金屬蛋白酶組織抑制因子-1（TIMP-1）、I型膠原（collagen I）的表達。結果 模型組血清TNF-α水平明顯高于空白對照組；與模型組比較，益母草組血清TNF-α水平顯著降低。模型組與空白對照組比較，MMP-13、TIMP-1明顯升高，collagen I表達上調；與模型組比較，益母草組MMP-13無明顯變化，TIMP-1明顯降低，collagen I表達下降。結論 益母草可能使產(chǎn)后炎癥子宮的TNF-α水平下降，下調TIMP-1的表達，啟動止血修復機制，并加快細胞外基質（ECM）降解，從而加速產(chǎn)后子宮復舊。

Objective To establish a postpartum uterine inflammation model in rats, and to investigate the internal mechanism of motherwort on postpartum uterine involution from the perspective of molecular biology. Methods Rats were randomly divided into four groups: control, model, motherwort, and Notoginseng groups. Uterine inflammation model was set up with Notoginseng as positive control, ELISA method was used to detect the serum tumor necrosis factor-α (TNF-α) levels in rats, rat uterus in various groups was observed by HE staining, and the expression of matrix metalloproteinase-13 (MMP-13), tissue inhibitor of metallo- proteinase type I (TIMP-1), and collagen I in uterine tissue was detected by immunohistochemical method. Results Serum TNF-α levels in the model group were significantly higher than that in the control group; Compared with the model group, serum TNF-α levels in motherwort group decreased significantly. Compared with control group, MMP-13 and TIMP-1 were significantly increased in the model group, and expression of collagen I was up-regulated. Compared with the model group, MMP-13 in motherwort group had no significant change, and expression of TIMP-1 and collagen I was significantly reduced. Conclusion This experiment confirms that motherwort could possibly reduce TNF-α levels in rats with postpartum uterine inflammation and the expression of TIMP-1 to start the repair mechanism of stop bleeding, and accelerate the degradation of extracellular matrix (ECM), so as to speed up postpartum uterine involution.

國家自然科學基金項目資助（30572405）