目的 研究氧誘導(dǎo)視網(wǎng)膜新生血管小鼠模型中視網(wǎng)膜血管內(nèi)皮細胞生長因子（VEGF）與視網(wǎng)膜新生血管的相關(guān)性。方法 通過建立氧誘導(dǎo)視網(wǎng)膜新生血管小鼠模型，分別于12、14、17、21 d齡隨機抽取2組幼鼠各6只，一側(cè)眼球測定視網(wǎng)膜VEGF水平，另一側(cè)眼球進行視網(wǎng)膜鋪片、ADP酶染色，考察其相關(guān)性。結(jié)果 模型組小鼠左眼球ADP酶染色結(jié)果顯示，模型組12 d齡小鼠血管迂曲、擴張、變形，隨時間延長血管變形加重，17 d時最嚴重，且視乳頭周圍出現(xiàn)大片無灌注區(qū)，視網(wǎng)膜周邊可見大量新生血管生成，到21 d時模型開始恢復(fù)。視網(wǎng)膜VEGF測定結(jié)果顯示，12 d齡模型小鼠VEGF開始升高，17 d達峰值，以后下降。結(jié)論 視網(wǎng)膜VEGF變化和視網(wǎng)膜血管形態(tài)具有很好的正相關(guān)性，17 d時新生血管形成最多。

Objective To study the correlation between retinal vascular endothelial growth factor (VEGF) level and retinal neovascularization in oxygen-induced retinal neovascularization model of mice. Methods The oxygen-induced retinal neovascularization model of mice was established and two groups of six mice for each group were randomly selected at 12, 14, 17, and 21 d age, respectively. The retinal VEGF in one side of the eyeballs was detected, the retinal sect serial sections and ADPase staining were carried out in the other side of the eyeballs, and the relevance was studied. Results In the model group, ADPase staining showed a pattern of pathological retinal neovascularization such as tortuous, dilated, and deformed blood vessels at 12 d age, and the deformation was aggravated with time. The most serious deformation was at 17 d age, and there were large nonperfused areas around discus opticus. A large number of new blood vessels were generated visibly in the peripheral retinal areas and the model began to recover at 21 d age. The retinal VEGF levels started to increase at 12 d age, reached the peak at 17 d age, and declined after that. Conclusion The retinal VEGF levels and the retinal vascular morphology have a positive correlation and neovascularization is the most serious at 17 d age.