目的 建立紫外分光光度法測(cè)定枸杞多酚提取物中的總多酚。方法 采用紫外分光光度法測(cè)定枸杞多酚提取物中總多酚，用水稀釋樣品，加入顯色劑Filin-Ciocalteu試劑，在760 nm處測(cè)定吸光度，用同樣方法測(cè)定并繪制標(biāo)準(zhǔn)曲線。結(jié)果 紫外分光光度法測(cè)定枸杞多酚提取物中總多酚的量分別為24.03%、26.38%、25.45%、28.27%、29.08%，線性范圍1～8 μg/mL，R²＝0.999 5。平均回收率為103.5%，RSD＜5%。結(jié)論 該方法操作簡(jiǎn)單，準(zhǔn)確可靠，適于枸杞多酚提取物中總多酚的測(cè)定。

Objective To establish UV-spectrophotometry to determine the content of total polyphenols in polyphenol extract from Lycium barbarum (PELB). Methods The content of total polyphenols in PELB was detected by using UV-spectrophotometry. The sample was diluted with water, chromogenic agent Filin-Ciocalteu was added, the absorbance was detected at 760 nm by UV-spectro- photometry, and with the same method standard curve was measured and drawn. Results The contents of total polyphenols in different batches of PELB were 24.03%, 26.38%, 25.45%, 28.27%, and 29.08%, respectively, the range of linearity was 1―8 μg/mL, and R² = 0.999 5. The average recovery was 103.5% and the RSD was less than 5%. Conclusion The method is proved to be simple, reliable, accurate, repeatable, and could be used to measure the content of total of polyphenols in PELB.