2=0.999 5。平均回收率為103.5%,RSD<5%。結(jié)論 該方法操作簡(jiǎn)單,準(zhǔn)確可靠,適于枸杞多酚提取物中總多酚的測(cè)定。;Objective To establish UV-spectrophotometry to determine the content of total polyphenols in polyphenol extract from Lycium barbarum (PELB). Methods The content of total polyphenols in PELB was detected by using UV-spectrophotometry. The sample was diluted with water, chromogenic agent Filin-Ciocalteu was added, the absorbance was detected at 760 nm by UV-spectro- photometry, and with the same method standard curve was measured and drawn. Results The contents of total polyphenols in different batches of PELB were 24.03%, 26.38%, 25.45%, 28.27%, and 29.08%, respectively, the range of linearity was 1―8 μg/mL, and R2 = 0.999 5. The average recovery was 103.5% and the RSD was less than 5%. Conclusion The method is proved to be simple, reliable, accurate, repeatable, and could be used to measure the content of total of polyphenols in PELB."/>

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首頁 > 過刊瀏覽>2012年第35卷第4期 >2012,35(4):273-275. DOI:10.7501/j.issn.0253-6376.[year].4.[sequence]
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枸杞多酚提取物中總多酚的測(cè)定

Determination of total polyphenols in polyphenol extract from Lycium barbarum

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