目的 研究不同提取方法所得丹參提取物中丹參酮Ⅱ_A與丹酚酸B在大鼠體內(nèi)的藥動學(xué)。方法 大鼠禁食12 h后，分別將加熱回流法、集成法、醇酸回流法和梯度滲漉法所得丹參提取物ig給予大鼠，對不同時刻血漿中的目標(biāo)成分進行定量測定，繪制出各提取方法下的藥時曲線并計算藥動學(xué)參數(shù)。結(jié)果 對于丹參酮Ⅱ_A而言，加熱回流法、集成法、梯度滲漉法和醇酸回流法所得提取物大鼠體內(nèi)的C_max分別為（0.25±0.03）、（0.35±0.06）、（0.32±0.04）、（0.24±0.02）mg/L；AUC為（101.97±12.95）、（130.46±18.83）、（128.67±16.63）、（100.11±13.76）mg?min/L。對于丹酚酸B而言，以上四種方法所得提取物在大鼠體內(nèi)的C_max分別為（7.17±0.97）、（11.98±1.75）、（10.24±1.05）、（12.16±2.08）mg/L；AUC為（697.31±80.32）、（833.41±96.53）、（719.39±102.41）、（906.96±125.87）mg?min/L。結(jié)論 對定量測定和藥動學(xué)參數(shù)進行統(tǒng)計分析，可知集成法所提取的丹參脂溶性成分和水溶性成分含量均比較高，并在體內(nèi)有較好的吸收，說明該法所得的提取物中丹參酮Ⅱ_A和丹酚酸B的質(zhì)量分數(shù)較高，提取效果可能較好。

Objective To study the pharmacokinetics of tanshinone II and salvianolic acid B extracted from Salvia miltiorrhiza by different methods in rats. Methods After fasted for 12 h, extracts from S. miltiorrhiza by different methods, such as heating reflux method, integrated method, gradient percolation, and acidity alcohol reflux method, were given to rats by ig administration, respectively. HPLC was used to determine the plasma concentration of active ingredients at different time points, then the concentration-time curve was mapped out and the pharmacokinetic parameters were calculated. Results For tanshinoneⅡ_A, the main pharmacokinetic parameters of heating reflux, integrated method, gradient percolation, and acidity alcohol reflux method were as follows: C_max were (0.25 ± 0.02), (0.35 ± 0.05), (0.32 ± 0.04), and (0.24 ± 0.02) mg/L; AUC were (101.97 ± 12.95), (130.46 ± 18.83), (128.67 ± 16.63), and (100.11 ± 13.76) (mg?min)/L. For salvianolic acid B, the main pharmacokinetic parameters of different extracting methods were as follows: C_max were (7.17 ± 0.97), (11.98 ± 1.75), (10.24 ± 1.05), (12.16 ± 2.08) mg/L; AUC were (697.31 ± 80.32), (833.41 ± 96.53), (719.39 ± 102.41), and (906.96 ± 125.87) mg?min/L. Conclusion The determination and statistical analysis of pharmacokinetic parameters demonstrated that compared with other methods, the extract of integrated method has higher content of liposoluble and water-soluble constituents, and good absorption in vivo, which means this method has better effect and the contents of tanshinone II and salvianolic acid B are higher.

黑龍江省留學(xué)歸國科學(xué)基金項目（LC2011C06）