目的 優(yōu)化小葉錦雞兒黃酮類成分的提取工藝，探討其對(duì)實(shí)驗(yàn)性腦缺血再灌注損傷大鼠的保護(hù)作用。方法 采用正交試驗(yàn)，以蘆丁作為對(duì)照，以總黃酮量為指標(biāo)考察小葉錦雞兒總黃酮的最佳提取工藝；采用大腦中動(dòng)脈阻斷（MCAO）制備大鼠局灶性腦缺血再灌注模型，ig給藥4周后，觀察小葉錦雞兒總黃酮對(duì)MCAO模型大鼠學(xué)習(xí)記憶能力的影響，并測(cè)定其血流變學(xué)各項(xiàng)指標(biāo)。結(jié)果 正交實(shí)驗(yàn)結(jié)果表明，各因素作用影響主次順序?yàn)椋阂汗瘫龋疽掖紳舛龋窘釙r(shí)間＞浸提溫度。小葉錦雞兒總黃酮能提高M(jìn)CAO模型大鼠的學(xué)習(xí)記憶能力，降低血漿黏度（η）、血小板聚集率（PAgT）、紅細(xì)胞聚集指數(shù)（EAI）、紅細(xì)胞剛性指數(shù)（IR），并增加紅細(xì)胞變形指數(shù)（DI）。結(jié)論 確定了總黃酮最佳提取工藝為乙醇濃度60%，浸提時(shí)間5 h，液固比40∶1，浸提溫度60 ℃。小葉錦雞兒總黃酮對(duì)腦缺血再灌注損傷具有腦保護(hù)作用，這可能與小葉錦雞兒總黃酮降低血黏度有關(guān)。

Objective To optimize the extraction technology of total flavonoid from Caragana microphylla (TFCM) and to explore the protective effect on cerebral ischemia-reperfusion injury in rats. Methods The optimum extraction technology was detected by orthogonal test with rutin as control and with the content of TFCM as an indicator. The middle-cerebral-artery-occlusion (MCAO) was used to induce the focal cerebral ischemia-reperfusion injury model of rats. The influence on the ability of learning and remembering and the effect on hemorheology were observed after ig administration with TFCM for four weeks. Results The result of orthogonal experiment indicated that the order of factors which affected the extraction was as follows: liquid-solid ratio > concentration of ethanol > extracting time > extracting temperature. TFCM could improve the ability of learning and remembering, decrease the levels of blood viscosity (η), platelet aggregation rate (PAgT), erythrocyte aggregation index (EAI), and erythrocyte rigidity index (IR), and increase the level of erythrocyte deformation index (DI). Conclusion The optimum extraction conditions are as follows: extracting for 5 h at 60 ℃ with the ratio of 60% ethanol to medicinal herbs (40 : 1). TFCM could protect the brain of the model rat with focal cerebral ischemia-reperfusion; It could be relevant to the role of decreasing blood viscosity.