[關(guān)鍵詞]
[摘要]
目的 研究竹黃菌的液體發(fā)酵最優(yōu)條件及生物學(xué)活性。方法 利用單因素實驗方法比較不同培養(yǎng)條件對竹黃菌生長和產(chǎn)竹紅菌素的影響,考察了竹黃菌發(fā)酵液抗菌和抗氧化的生物學(xué)活性。結(jié)果 竹黃菌的較優(yōu)培養(yǎng)條件為:蔗糖2.0%,葡萄糖3.0%,土豆20%,pH 7.0,菌種活化后放置72 h接種;竹黃菌發(fā)酵液對大腸桿菌、金黃色葡萄球菌、枯草芽孢桿菌、釀酒酵母、黑曲霉和黑根霉等均表現(xiàn)出了較強的抗性,對氧自由基和·OH的清除率分別為84.2%和76.32%。結(jié)論 竹黃菌的液體發(fā)酵條件簡單,發(fā)酵產(chǎn)物具有一定的廣譜抗菌性能和抗氧化活性,在養(yǎng)殖業(yè)中有著良好的應(yīng)用前景。
[Key word]
[Abstract]
Objective To investigate the optimal conditions for liquid fermentation of Shiraia bambusicola and its bio-activities. Methods With the method of single-factor-test, the fermentation conditions of S. bambusicola and the production of hypocrellin were optimized, and the antibiosis and anti-oxidative activities were investigated further. Results The results showed that the optimized culture conditions were as follows: sugar 2.0%, glucose 3.0%, potato 20%, initial pH 7.0, and the spawn was inoculated after being preserved for 72 h. The krausen of S. bambusicola was resistant to Escherichia coli, Bacillus subtilis, Staphyloccocus aureus, Saccharomyces cerevisiae, Aspergullus niger, and Rhizopus niger, and to the oxygen radicals and ?OH with the clearance rate of 84.2% and 76.32%, respectively. Conclusion The strain of S. bambusicola is cultivated with simple conditions. The krausen is a potential additive in the cultivation with excellent anti-oxidative activities and broad antibiosis.
[中圖分類號]
[基金項目]
義烏市科研計劃項目(10-3-03)