目的 建立HPLC內(nèi)標多控法測定枳實藥材中黃酮類成分的方法，并對測定結(jié)果進行不確定度評定。方法 以柚皮苷為內(nèi)標，建立柚皮苷與橙皮苷、新橙皮苷的相對校正因子，計算枳實中黃酮類成分的含量，再根據(jù)數(shù)學(xué)模型，對測定過程中引入的不確定度進行評估，由此計算測定結(jié)果不確定度。結(jié)果 采用本方法測定枳實中柚皮苷與橙皮苷、新橙皮苷含量的擴展不確定度分別為W×2.94%、W×3.49%、W×3.34%。結(jié)論 建立的不確定度評定法可為內(nèi)標多控法測定中藥有效成分的不確定度分析提供參考。

Objective To establish an HPLC internal standard multi-control method for the determination of flavonoids in Aurantii Immaturus Fructus and to evaluate the uncertainty of the results. Methods Using naringin as internal standard, the relative correction factor of naringin to hesperidin and neohesperidin was established. The contents of flavonoids in Aurantii Immaturus Fructus were determined. Through the mathematical mode, the factors affecting the uncertainty were deduced and evaluated. Results The expanded standard uncertainties for the determined contents of naringin, hesperidin, and neohesperidin in Aurantii Immaturus Fructus were W × 2.94%, W × 3.49%, and W × 3.34%, respectively. Conclusion The established mathematic model is applicable to the uncertainty analysis of HPLC internal standard multi-control method in the determination of active components in Chinese materia medica.