目的 采用頂空氣相色譜法測定人參莖葉皂苷中大孔樹脂殘留物的質(zhì)量濃度。方法 色譜柱初始溫度60 ℃，保持22 min，以50 ℃/min升至200 ℃，保持6 min，氫火焰離子檢測器溫度為260 ℃，進(jìn)樣口溫度220 ℃；用質(zhì)量分?jǐn)?shù)為40%的N, N-二甲基甲酰胺作溶劑，載氣為氮氣。結(jié)果 正己烷、苯、甲苯、對二甲苯、苯乙烯、對二乙基苯6種大孔樹脂殘留物在各自的質(zhì)量濃度范圍內(nèi)呈良好的線性關(guān)系，且回收率符合要求。結(jié)論 該方法操作簡便快速，靈敏度高，準(zhǔn)確度好，可作為人參莖葉總皂苷中大孔樹脂有機(jī)溶劑殘留量的測定方法。

Objective To establish a headspace gas chromatography method for the determination of macroporous resin residues in the saponins extracted from the leaves of Panax ginseng. Methods The initial column temperature was 60 ℃ (for 22 min), and increased to 200 ℃ at a rate of 50 ℃/min (for 6 min); The injector temperature was set at 220 ℃ and the temperature of FID detector was set at 260 ℃; The solvent was 40% N, N-dimethyl formamide (DMF); The carrier gas was nitrogen. Results All the residues including n-hexane, benzene, toluene, 1,4-xylene, styrene, and 1,4-diethyl-benzene had good linearities and the recoveries in their concentration ranges, respectively. Conclusion The method is simple, quick, accurate, and sensitive. It could be used for the determination of macroporous resin residues in the saponins from the leaves of P. ginseng.