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[摘要]
目的 篩選丹參中對(duì)酪氨酸酶活性具有抑制作用的有效成分。方法 基于受試物抑制酪氨酸酶催化底物L-多巴的反應(yīng)原理,將丹參經(jīng)80%乙醇提取后依次經(jīng)石油醚、氯仿、醋酸乙酯、飽和正丁醇和水5種不同極性溶劑萃取,分別檢測各提取部位對(duì)酪氨酸酶活性的抑制作用;并通過UPLC-MS/MS技術(shù)鑒定活性最強(qiáng)提取部位化學(xué)成分,結(jié)合分子對(duì)接技術(shù)預(yù)測各成分潛在活性,并通過酶學(xué)檢測驗(yàn)證。結(jié)果 丹參氯仿萃取層具有較強(qiáng)的酪氨酸酶抑制活性。其生物活性與丹參素、原兒茶醛等6種成分有關(guān),酶學(xué)檢測驗(yàn)證結(jié)果與篩選結(jié)果一致。結(jié)論 丹參中丹參素和原兒茶醛等成分對(duì)酪氨酸酶活性具有顯著的抑制作用。
[Key word]
[Abstract]
Objective To screen the active components in Salvia miltiorrhiza with the inhibitory activity against tyrosinase. Methods The method of L-Dopa oxidization reaction was employed to detect the tyrosinase activity. S. miltiorrhiza was first extracted by 80% ethanol and then extracted by PE, CHCl3, EtOAc, n-BuOH, and H2O. The inhibition of the different fractions on tyrosinase was detected. UPLC-MS/MS was used to analyze and identify the active components in the fraction with the strongest inhibition. Molecular docking was used to predict the potential activity and enzymological method was used to validate their efficacy components. Results The chloroform fraction showed a strong inhibition on tyrosinase activity. The bioactivities had the relationship with six components, such as tanshinol and protocatechualdehyde, etc. Enzymological method showed the same results with the screening. Conclusion Tanshinol, protocatechualdehyde, and so on in S. Miltiorrhiza have the significant inhibition on tyrosinase.
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