3, EtOAc, n-BuOH, and H2O. The inhibition of the different fractions on tyrosinase was detected. UPLC-MS/MS was used to analyze and identify the active components in the fraction with the strongest inhibition. Molecular docking was used to predict the potential activity and enzymological method was used to validate their efficacy components. Results The chloroform fraction showed a strong inhibition on tyrosinase activity. The bioactivities had the relationship with six components, such as tanshinol and protocatechualdehyde, etc. Enzymological method showed the same results with the screening. Conclusion Tanshinol, protocatechualdehyde, and so on in S. Miltiorrhiza have the significant inhibition on tyrosinase."/>