目的 研究溫郁金內(nèi)生真菌的抗炎抗氧化活性，旨在獲得有良好抗炎抗氧化活性的菌株，以尋找新型抗炎抗氧化活性物質(zhì)。方法 采用二甲苯致小鼠耳廓腫脹及醋酸致小鼠腹腔毛細(xì)血管通透性的動(dòng)物模型對已分離的50株溫郁金內(nèi)生真菌發(fā)酵產(chǎn)物進(jìn)行抗炎活性篩選；采用DPPH法測試內(nèi)生真菌的抗氧化活性。結(jié)果 有21株內(nèi)生真菌能夠抑制二甲苯誘導(dǎo)的小鼠耳腫脹，并且對醋酸所致小鼠腹腔毛細(xì)血管通透性有降低作用，其中菌株E3、E8、E17、E24、E37、E46的抑制作用最為顯著（P＜0.01），作用與萘普生相當(dāng)；具有抗氧化活性的菌株8株。并且其抗氧化活性存在劑量效應(yīng)關(guān)系，其中活性菌株E2、E17、E37在質(zhì)量濃度為140 μg/mL時(shí)對DPPH自由基清除率都在90%以上。結(jié)論 溫郁金含有抗炎抗氧化活性的內(nèi)生真菌，菌株E2、E3、E8、E17、E24、E37、E46具有良好的抗炎抗氧化效果，具有一定的研究開發(fā)前景。

Objective To study the anti-inflammatory and anti-oxidant activities of endophytic fungi from medicinal plant Curcuma wenyujin, to obtain the strains with good anti-inflammatory and anti-oxidant activities, and to find the new substances with anti-inflammatory and anti-oxidant activities. Methods The anti-inflammatory activities of 50 strains from the isolated endophytic fungi were tested by animal models with ear swelling induced by xylene and peritoneal capillary permeability induced by acetic acid in mice; DPPH method was used to evaluate the anti-oxidant activities of endophytic fungi. Results Twenty-one strains of endophytic fungi could inhibit ear swell induced by xylene in mice and reduce peritoneal capillary permeability of mice induced by acetic acid. Compared with the group of physiological saline, the groups of E3, E8, E17, E24, E37, and E4 had the significant inhibitory effects (P< 0.01), which was the same as Naproxen; Eight strains of endophytic fungi had the anti-oxidant activity. There was a dose-effect relationship between the fermentation products and the anti-oxidant activity, and their free radical scavenging rates against DPPH were above 90% when the concentration of E2, E17, and E37 were at 140 μg/mL. Conclusion Some endophytic fungi from C. wenyujin have the anti-inflammatory and anti-oxidant activities, strains E2, E3, E8, E17, E24, E37, and E46 show the significant effect on the anti-inflammatory and anti-oxidant activities, which has a perspective in research and development.

吉林農(nóng)業(yè)大學(xué)科研啟動(dòng)基金資助項(xiàng)目（201110）