目的 觀察參附注射液對大鼠腦缺血再灌注后磷酸果糖激酶（PFK）表達(dá)的影響，探討參附注射液對腦的保護(hù)機(jī)制。方法 清潔級雄性SD大鼠90只，隨機(jī)分為假手術(shù)（Sham）組、腦缺血再灌注（IR）組、參附注射液預(yù)處理（SFI組），每組30只。采用線栓法制備大鼠大腦中動脈閉塞（MCAO）后再灌注模型，TTC染色測腦梗死面積，并檢測PFK活性及PFK mRNA和蛋白的表達(dá)。結(jié)果 參附注射液能明顯減少腦缺血再灌注大鼠腦梗死灶的面積。IR組與Sham組比較，PFK活性明顯降低（P＜0.05），PFK mRNA及蛋白的表達(dá)明顯增加（P＜0.05）；SFI組PFK mRNA及蛋白的表達(dá)水平及活性明顯高于IR組（P＜0.05）。結(jié)論 參附注射液對腦缺血再灌注大鼠腦組織有保護(hù)作用，其機(jī)制可能與上調(diào)PFK表達(dá)及活性有關(guān)。

Objective To investigate the effect of Shenfu Injection (SFI) on the expression of phosphofructokinase (PFK) in rats after cerebral ischemia-reperfusion injury and to discuss its protective mechanism on brain. Methods Male SD rats were randomly divided into three groups, Sham, cerebral ischemic reperfusion (IR), and SFI-pretreated (SFI) groups, with 30 rats in each group. Reversible middle cerebral artery occlusion (MCAO) model was produced by intraluminal suture technique. The infarct area of brain was measured by 2,3,5-triphenyl tetrazolium chloride (TTC) staining method; The PFK activity and the expression of PFK mRNA and protein were detected, respectively. Results SFI could significantly decrease the infarct areas of brain with MCAO in rats. Compared with those in Sham group, the PFK activity decreased (P < 0.05) and the expression of PFK mRNA and protein increased in IR group (P < 0.05). The PFK activity and the expression of PFK mRNA and protein were significantly increased in SFI group compared with IR group (P < 0.05). Conclusion SFI has the protective effects on the brain injury induced by ischemia. Its mechanism may be correlated to up-regulating the expression and activity of PFK.

四川省科技廳資助項(xiàng)目（2010JY0094）