目的 建立有效、簡便的方法對腋臭粉及含汞制劑進行微生物限度檢查。方法 通過加入中和劑硫代乙醇酸鈉來中和氧化汞，以達到破壞供試品抑菌活性的目的。細菌數測定采用離心沉淀-中和-薄膜過濾法,霉菌和酵母菌數測定以及控制菌檢查采用中和-培養(yǎng)基稀釋法。結果 細菌、霉菌和酵母菌計數方法驗證實驗中，實驗組和稀釋劑對照組的菌回收率均不低于70%，控制菌檢查結果符合要求。結論 該方法有效、簡便，可行性強，可作為腋臭粉及含汞制劑的微生物限度檢查方法。

Objective To establish an effective method for the microbiological limit test of Yexiu Powder and mercury containing preparations. Methods Sodium thioglycollate was used to neutralize mercuric oxide so that the antibacterial activity in the test was destroyed. Centrifugal sedimentation-neutralization-membrane filtration method was used for the validation test of bacteria count, and neutralization-medium dilution method was used for the validation test of mold and yeast count and control bacteria inspection. Results In the validation tests of bacterial, mold and yeast count, the microbial recovery rates were not less than 70% both in test group and in diluted control group. In the tests of specified microbial species, the results met the standard. Conclusion The method is effective and feasible, which could be used for the microbiological limit test for Yexiu Powder and mercury containing preparations.