目的 研究染料木黃酮在Caco-2單細(xì)胞層模型上的吸收特征。方法 將染料木黃酮的細(xì)胞樣品經(jīng)高速離心后取上清液，以乙腈-醋酸銨水溶液為流動(dòng)相，以睪丸酮為內(nèi)標(biāo)，采用超高效液相色譜（ultra performance liquid chromatography，UPLC）法檢測(cè)?？疾鞎r(shí)間、pH值等因素對(duì)染料木黃酮吸收的影響，測(cè)定透過(guò)Caco-2單細(xì)胞層的染料木黃酮濃度并計(jì)算其表觀滲透系數(shù)（P_app）。結(jié)果 Caco-2細(xì)胞對(duì)染料木黃酮的吸收在1 h內(nèi)呈線性，藥物攝取時(shí)間定為1 h；pH值對(duì)染料木黃酮的吸收沒(méi)有顯著影響。染料木黃酮的線性范圍為0.625～40 μmol/L，日間、日內(nèi)精密度均小于15%。P_app從基頂側(cè)（apical，AP）到基底側(cè)（basolateral，BL）為1.51×10^?5 cm/s，從BL側(cè)到AP側(cè)為1.84×10^?5 cm/s，其滲透系數(shù)P_app（BL-AP）/P_app（AP-BL）為1.22。結(jié)論 染料木黃酮在腸道的吸收主要以被動(dòng)擴(kuò)散為主，UPLC檢測(cè)方法簡(jiǎn)單、靈敏度高，可用于研究染料木黃酮在Caco-2單細(xì)胞層模型上的吸收特征。

Objective To study the absorptive characteristics of genistein in Caco-2 cell monolayer model. Methods The genistein cell samples underwent high speed centrifugation, then the supernatant was collected and detected by UPLC method with acetonitrile and ammonium acetate water solution as mobile phase and testosterone as internal standard. The effects of time and pH value on genistein were investigated. The concentration of genistein across the Caco-2 cell monolayer model was determined and the apparent permeability coefficient (P_app) of genistein was calculated. Results The uptake of genistein in Caco-2 cells showed a linear range in 1 h and the uptake time was fixed at 1 h. pH value showed no significant influence on the absorption of genistein. The linear range of genistein was 0.625—40 μmol/L, with intra- and inter-day precision less than 15%. P_app (AP－BL) from apical side (AP) to basolateral side (BL) was 1.51 × 10^?5 cm/s, whereas P_app (BL-AP) from BL to AP was 1.84 × 10^?5 cm/s, and the efflux ratio of P_app (BL-AP)/ P_app (AP-BL) was 1.22. Conclusion Passive diffusion is the major process for the transport of genistsein across the intestine. The UPLC method is simple and sensitive, and it is applicable for the study on absorptive characteristics of genistein in Caco-2 cell monolayer model.