18（250 mm×4.6 mm，5 μm）色譜柱，流動相為乙睛-甲醇-1%醋酸（10∶15∶75），體積流量1.0 mL/min，檢測波長334 nm，柱溫為室溫。結果 松果菊苷進樣量在0.46～18.40 μg內與峰面積呈良好的線性關系（r＝0.999 9）；平均回收率為101.11%，RSD為2.09%（n＝6）。結論 采用RP-HPLC測定短筒兔耳草藥材中松果菊苷的含量，該方法準確、快速、簡便，重復性好，適用于短筒兔耳草中松果菊苷的質量控制。;Objective To develop a RP-HPLC method for the determination of echinacoside in Lagotis brevituba. Methods L. brevituba was extracted with the mobile phase by ultrasound extraction and then determined by RP-HPLC. The chromatograph was carried out on Alltech Prevail C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-methanol-1% acetic acid (10:15:75) as the mobile phase at the flow rate of 1.0 mL/min, and the detection wavelength was set at 334 nm at room temperature. Results The linear range of echinacoside was 0.46—18.40 μg (r=0.999 9). The average recovery of echinacoside was 101.11% and RSD was 2.09% (n=6). Conclusion The method is accurate, quick, simple, and reproducible, and it could be used for the quality control of L. brevituba."/>

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首頁 > 過刊瀏覽>2013年第36卷第2期 >2013,36(2):132-134. DOI:10.7501/j.issn.1674-6376.2013.02.013
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RP-HPLC法測定短筒兔耳草中松果菊苷

Determination of echinacoside in Lagotis brevituba by RP-HPLC

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    [關鍵詞]
    [摘要]
    目的 建立短筒兔耳草中松果菊苷的RP-HPLC測定方法。方法 短筒兔耳草經流動相超聲提取后，用RP-HPLC法測定。采用Alltech Prevail C18（250 mm×4.6 mm，5 μm）色譜柱，流動相為乙睛-甲醇-1%醋酸（10∶15∶75），體積流量1.0 mL/min，檢測波長334 nm，柱溫為室溫。結果 松果菊苷進樣量在0.46～18.40 μg內與峰面積呈良好的線性關系（r＝0.999 9）；平均回收率為101.11%，RSD為2.09%（n＝6）。結論 采用RP-HPLC測定短筒兔耳草藥材中松果菊苷的含量，該方法準確、快速、簡便，重復性好，適用于短筒兔耳草中松果菊苷的質量控制。
    [Key word]
    [Abstract]
    Objective To develop a RP-HPLC method for the determination of echinacoside in Lagotis brevituba. Methods L. brevituba was extracted with the mobile phase by ultrasound extraction and then determined by RP-HPLC. The chromatograph was carried out on Alltech Prevail C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-methanol-1% acetic acid (10:15:75) as the mobile phase at the flow rate of 1.0 mL/min, and the detection wavelength was set at 334 nm at room temperature. Results The linear range of echinacoside was 0.46—18.40 μg (r=0.999 9). The average recovery of echinacoside was 101.11% and RSD was 2.09% (n=6). Conclusion The method is accurate, quick, simple, and reproducible, and it could be used for the quality control of L. brevituba.
    [中圖分類號]
    [基金項目]
    《中國藥典》2010版一部標準研究項目（YS-204）
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