18(250 mm×4.6 mm,5 μm)色譜柱,流動相為乙睛-甲醇-1%醋酸(10∶15∶75),體積流量1.0 mL/min,檢測波長334 nm,柱溫為室溫。結果 松果菊苷進樣量在0.46~18.40 μg內與峰面積呈良好的線性關系(r=0.999 9);平均回收率為101.11%,RSD為2.09%(n=6)。結論 采用RP-HPLC測定短筒兔耳草藥材中松果菊苷的含量,該方法準確、快速、簡便,重復性好,適用于短筒兔耳草中松果菊苷的質量控制。;Objective To develop a RP-HPLC method for the determination of echinacoside in Lagotis brevituba. Methods L. brevituba was extracted with the mobile phase by ultrasound extraction and then determined by RP-HPLC. The chromatograph was carried out on Alltech Prevail C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-methanol-1% acetic acid (10:15:75) as the mobile phase at the flow rate of 1.0 mL/min, and the detection wavelength was set at 334 nm at room temperature. Results The linear range of echinacoside was 0.46—18.40 μg (r=0.999 9). The average recovery of echinacoside was 101.11% and RSD was 2.09% (n=6). Conclusion The method is accurate, quick, simple, and reproducible, and it could be used for the quality control of L. brevituba."/>