目的 建立短筒兔耳草中松果菊苷的RP-HPLC測定方法。方法 短筒兔耳草經(jīng)流動相超聲提取后，用RP-HPLC法測定。采用Alltech Prevail C₁₈（250 mm×4.6 mm，5 μm）色譜柱，流動相為乙睛-甲醇-1%醋酸（10∶15∶75），體積流量1.0 mL/min，檢測波長334 nm，柱溫為室溫。結(jié)果 松果菊苷進樣量在0.46～18.40 μg內(nèi)與峰面積呈良好的線性關(guān)系（r＝0.999 9）；平均回收率為101.11%，RSD為2.09%（n＝6）。結(jié)論 采用RP-HPLC測定短筒兔耳草藥材中松果菊苷的含量，該方法準確、快速、簡便，重復(fù)性好，適用于短筒兔耳草中松果菊苷的質(zhì)量控制。

Objective To develop a RP-HPLC method for the determination of echinacoside in Lagotis brevituba. Methods L. brevituba was extracted with the mobile phase by ultrasound extraction and then determined by RP-HPLC. The chromatograph was carried out on Alltech Prevail C₁₈ column (250 mm × 4.6 mm, 5 μm) with acetonitrile-methanol-1% acetic acid (10:15:75) as the mobile phase at the flow rate of 1.0 mL/min, and the detection wavelength was set at 334 nm at room temperature. Results The linear range of echinacoside was 0.46—18.40 μg (r=0.999 9). The average recovery of echinacoside was 101.11% and RSD was 2.09% (n=6). Conclusion The method is accurate, quick, simple, and reproducible, and it could be used for the quality control of L. brevituba.

《中國藥典》2010版一部標準研究項目（YS-204）