目的 選用閃式提取法對(duì)三七果進(jìn)行提取工藝的考察，并通過采用高效液相色譜法對(duì)三七果所含的主要單體皂苷進(jìn)行測(cè)定，優(yōu)選出最佳提取方式。方法 采用閃式提取法提取三七果中的總皂苷，制備出9個(gè)供試品；以人參皂苷Rb₁為對(duì)照品，應(yīng)用HPLC-UV法測(cè)定各個(gè)供試品中的單體皂苷。色譜柱條件：Hypersil C₁₈柱（200 mm×4.6 mm，5 μm），流動(dòng)相為乙腈-水梯度洗脫（0 min，20∶80→30 min，45∶55→48 min，70∶30→50 min，80∶20→60 min，100∶0）；檢測(cè)波長(zhǎng)203 nm；體積流量1.0 mL/min。結(jié)果 閃式提取可得到較高收率的總皂苷，且單體皂苷人參皂苷Rb₁的量較高。結(jié)論 閃式提取法速度快，效率高，耗材少，適用于三七果總皂苷的提取和制備。

Objective To extract the active components from the fruits of Panax notoginseng (FPN) using optimal flash extraction technology and to determine the major monomer saponins in FPN using HPLC method. Methods The flash extraction technology was used to extract the total saponins including nine samples from FPN. The monomer saponins in each sample were determined by HPLC-UV with ginsengside Rb₁ as reference substance. The chromatographic conditions were as follows: Hypersil C₁₈ column (200 mm × 4.6 mm, 5 μm), acetonitrile-warter (0 min, 20:80→30 min, 45:55→48 min, 70:30→50 min, 80:20→60 min, 100:0); The detective wavelength was set at 203 nm; The flow rate was set at 1.0 mL/min. Results Flash extraction technology is the efficient method with the high yield of active saponins. Conclusion Flash extraction technology is a rapid, effective, and economical method used for extracting the active saponins from the different parts of P. notoginseng.

遼寧省天然藥物現(xiàn)代分離與工業(yè)化制備工程技術(shù)研究中心資助項(xiàng)目(2006-19-10)