1為對(duì)照品,應(yīng)用HPLC-UV法測(cè)定各個(gè)供試品中的單體皂苷。色譜柱條件:Hypersil C18柱(200 mm×4.6 mm,5 μm),流動(dòng)相為乙腈-水梯度洗脫(0 min,20∶80→30 min,45∶55→48 min,70∶30→50 min,80∶20→60 min,100∶0);檢測(cè)波長(zhǎng)203 nm;體積流量1.0 mL/min。結(jié)果 閃式提取可得到較高收率的總皂苷,且單體皂苷人參皂苷Rb1的量較高。結(jié)論 閃式提取法速度快,效率高,耗材少,適用于三七果總皂苷的提取和制備。;Objective To extract the active components from the fruits of Panax notoginseng (FPN) using optimal flash extraction technology and to determine the major monomer saponins in FPN using HPLC method. Methods The flash extraction technology was used to extract the total saponins including nine samples from FPN. The monomer saponins in each sample were determined by HPLC-UV with ginsengside Rb1 as reference substance. The chromatographic conditions were as follows: Hypersil C18 column (200 mm × 4.6 mm, 5 μm), acetonitrile-warter (0 min, 20:80→30 min, 45:55→48 min, 70:30→50 min, 80:20→60 min, 100:0); The detective wavelength was set at 203 nm; The flow rate was set at 1.0 mL/min. Results Flash extraction technology is the efficient method with the high yield of active saponins. Conclusion Flash extraction technology is a rapid, effective, and economical method used for extracting the active saponins from the different parts of P. notoginseng."/> 1;HPLC;fruits of Panax notoginseng; flash extraction; orthogonal test; ginsengside Rb1; HPLC"/>