[關(guān)鍵詞]
[摘要]
目的 以黃連為對象探討中藥譜效關(guān)系研究新方法。方法 建立優(yōu)化基于ATP生物發(fā)光的微量抑菌檢測方法,與微量稀釋法抑菌結(jié)果進行相關(guān)性考察。以所建立的抑菌檢測方法檢測黃連抑菌活性,整合黃連的化學(xué)與抑菌活性圖譜,探討黃連化學(xué)組分與活性之間的關(guān)系。結(jié)果 所建立的基于ATP生物發(fā)光抑菌檢測法與微量稀釋法相關(guān)性良好,分別檢測大腸桿菌和金黃色葡萄球菌所得相關(guān)系數(shù)r值分別為0.998和0.957。黃連譜效相關(guān)研究結(jié)果顯示,黃連中除主要有效成分黃連素外,還可能含有微量高效抑菌成分。結(jié)論 基于ATP生物發(fā)光的抑菌活性檢測方法適用于中藥抑菌活性的譜效關(guān)系研究,為中藥質(zhì)量控制和藥效評價提供新的技術(shù)手段。
[Key word]
[Abstract]
Objective To explore the new methods for the research of spectrum-effect relationship of Chinese materia medica (CMM), taking Coptidis Rhizoma as an object. Methods The trace bacteriostatic detection method based on ATP bioluminescence was established and optimized, and the correlation of the results between ATP bioluminescence and microdilution method was investigated. The antibacterial activity of Coptidis Rhizoma was detected by the antibacterial detection method. and the chemical and activity profiles of Coptidis Rhizoma were integrated to discuss the relationship between the chemical composition and the activities. Results The results obtained by ATP bioluminescence assay correlated well with those by microdilution method, the r values of Escherichia coli and Staphylococcus aureus were 0.998 and 0.957, respectively. The integration activity spectra showed that except berberine there may be other substances with the high antibacterial activity in Coptidis Rhizoma. Conclusion ATP bioluminescence could be used in the study on the spectrum-effect relationship of antibacterial activity in CMM, in order to provide a new method for the quality control and effect evaluation of CMM.
[中圖分類號]
[基金項目]
國家自然科學(xué)基金資助項目(81173471);江蘇高校優(yōu)勢學(xué)科建設(shè)工程項目資助