目的 建立二-(2, 4-二氯苯甲酰異羥肟酸) 二苯基合錫（DPDCT）在大鼠血漿中蛋白結(jié)合率的測定方法。方法 采用HPLC法測定大鼠血漿中藥物總濃度及游離藥物濃度，結(jié)合平衡透析法測定血漿蛋白結(jié)合率。結(jié)果 DPDCT在質(zhì)量濃度為0.2、1.0、5.0 μg/mL時(shí)與大鼠的血漿蛋白結(jié)合率分別為（58.9±2.0）%、（57.4±1.2）%和（58.6±0.8）%。不同濃度組血漿蛋白結(jié)合率差異無統(tǒng)計(jì)學(xué)意義。結(jié)論 該方法靈敏度高，重復(fù)性好，操作簡單，能夠滿足生物樣品分析的要求。DPDCT具有中等強(qiáng)度的血漿蛋白結(jié)合率。

Objective To develop a method for the determination of the protein binding rate of diphenylpi-(2, 4-dichlorbenzohydroxamato) tin (IV) (DPDCT) in rat plasma. Methods The HPLC combined with equilibrium dialysis was carried out to determine the plasma protein binding rate of DPDCT. Results The plasma protein binding rates in rats were(58.9 ± 2.0)%, (57.4 ± 1.2)%, and (58.6 ± 0.8)% when the concentration of DPDCT was 0.2, 1.0, and 5.0 μg/mL, respectively. There was no significant difference in all groups. Conclusion The method is simple, rapid, accurate, and sensitive, and it could meet the requirement for analysis method of biological samples. DPDCT has medium plasma protein binding rates.

國家“重大新藥創(chuàng)制”科技重大專項(xiàng)（2009ZX09103-104）；國家自然科學(xué)基金資助項(xiàng)目（30973603）；山西省歸國人員基金（2010-54）；山西醫(yī)科大學(xué)08博士啟動基金